Invasion of Candida albicans Correlates with Expression of Secreted Aspartic Proteinases during Experimental Infection of Human Epidermis
| Autor: | Carolin Schackert, Elfriede Januschke, Hans C. Korting, Bernhard Hube, Martin Schaller |
|---|---|
| Rok vydání: | 2000 |
| Předmět: |
Hyphal growth
RT-PCR Germ tube Virulence Dermatology Biology Biochemistry Microbiology chemistry.chemical_compound immunoelectron microscopy Candida albicans Pepstatins medicine Aspartic Acid Endopeptidases Humans Protease Inhibitors Stratum spinosum Microscopy Immunoelectron Molecular Biology cutaneous candidiasis Candidiasis Cell Biology biology.organism_classification Corpus albicans medicine.anatomical_structure chemistry Mutation Epidermis Keratinocyte Pepstatin |
| Zdroj: | Journal of Investigative Dermatology. 114(4):712-717 |
| ISSN: | 0022-202X |
| DOI: | 10.1046/j.1523-1747.2000.00935.x |
| Popis: | Secreted aspartic proteinases (Saps) encoded by 10 genes of Candida albicans are important virulence factors for different types of candidiasis. Distinct SAP genes have previously been shown to contribute to tissue damage in a model of oral candidiasis. In this study a progressive SAP expression in the order SAP1 and SAP2 > SAP8 > SAP6 > SAP3 was observed in an in vitro model of cutaneous candidiasis based on reconstituted human epidermis. Transcripts of SAP1 and SAP2 were detected during initial invasion of the stratum corneum by C. albicans. Deeper, extensive penetration of the corneal layer was accompanied by additional SAP8 mRNA. SAP6 expression occurred concomitantly with germ tube formation and extensive hyphal growth in the strata granulosum, spinosum, and basale. Ultrastructural studies using specific polyclonal antibodies directed against the gene products of SAP1–3 and SAP4–6 revealed predominant expression of Sap1–3. The protective effect of the aspartic proteinase inhibitor pepstatin A during infection of the epidermis and an attenuated virulence phenotype of SAP -deficient mutants suggest that the observed SAP expression correlates with tissue damage in the skin. |
| Databáze: | OpenAIRE |
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