Structure of the O-polysaccharide leads to classification ofProteus penneri31 inProteusserogroup O19

3)-D-GlcNAc shared with a number of other Proteus O-polysaccharides could not provide any significant cross-reactivity of the corresponding LPS with rabbit polyclonal O-antiserum against P. penneri 31. -->
ISSN: 1574-695X
0928-8244
DOI: 10.1016/s0928-8244(03)00205-0
Přístupová URL adresa: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e520269a882bf0ef07c1fee976f8ef08
https://doi.org/10.1016/s0928-8244(03)00205-0
Rights: OPEN
Přírůstkové číslo: edsair.doi.dedup.....e520269a882bf0ef07c1fee976f8ef08
Autor: Anna N. Kondakova, Zygmunt Sidorczyk, Sof'ya N. Senchenkova, Yuriy A. Knirel, Krystyna Zych, Alexander S. Shashkov, Agnieszka Zabłotni
Rok vydání: 2003
Předmět:
Zdroj: FEMS Immunology & Medical Microbiology. 39:73-79
ISSN: 1574-695X
0928-8244
DOI: 10.1016/s0928-8244(03)00205-0
Popis: O-polysaccharide was obtained by mild acid degradation of the lipopolysaccharide (LPS) of Proteus penneri strain 31. Sugar and methylation analyses along with NMR spectroscopic studies, including 2D 1H,1H COSY, TOCSY, ROESY, 1H,13C and 1H,31P HMQC experiments, demonstrated the following structure of the polysaccharide: [carbohydrate structure: see text] where FucNAc is 2-acetamido-2,6-dideoxygalactose and EtnP is 2-aminoethyl phosphate. The polysaccharide studied has the same carbohydrate backbone as the O-polysaccharide of Proteus vulgaris O19. Based on this finding and close serological relatedness of the LPS of the two strains, it is proposed to classify P. penneri 31 in Proteus serogroup O19 as an additional subgroup. In contrast, D-GlcNAc6PEtn and alpha-L-FucNAc-(1-->3)-D-GlcNAc shared with a number of other Proteus O-polysaccharides could not provide any significant cross-reactivity of the corresponding LPS with rabbit polyclonal O-antiserum against P. penneri 31.
Databáze: OpenAIRE
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