β-keratin expression in avian tongue cell aggregates
| Autor: | Wayne Carver, Loren W. Knapp, Roger H. Sawyer |
|---|---|
| Rok vydání: | 1990 |
| Předmět: |
Cell
Chick Embryo Biology Tongue Keratin Gene expression medicine Animals Cell Aggregation chemistry.chemical_classification integumentary system Cell Differentiation Embryo General Medicine Anatomy Epithelium In vitro Cell biology medicine.anatomical_structure Gene Expression Regulation chemistry Multigene Family Keratins Immunohistochemistry Animal Science and Zoology |
| Zdroj: | Journal of Experimental Zoology. 256:333-338 |
| ISSN: | 1097-010X 0022-104X |
| DOI: | 10.1002/jez.1402560313 |
| Popis: | Rotation-mediated aggregation was used to test the capacity of early embryonic tongue cells from different regions of the tongue to reorganize histotypically and terminally differentiate in vitro. Cells dissociated from the anterior ventral (AV) or posterior ventral (PV) surface of the 12 day embryonic tongue were cultured 6 days, fixed, and embedded in paraffin. Indirect immunofluorescence microscopy was used to demonstrate the occurrence and distribution of beta-keratins, the presence of which represented regional and differentiation specific markers of late tongue development. Aggregates of AV cells were organized into a beta-keratin-producing stratified epithelium. Similar PV aggregates formed only an alpha-keratin-producing stratified epithelium. The epithelial cells of these tongue tissues constructs expressed alpha- and beta-keratins in a manner consistent with the temporal and histotypical expression of keratins observed in vivo. |
| Databáze: | OpenAIRE |
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