Photodynamic therapy corrects abnormal cancer-associated gene expression observed in actinic keratosis lesions and induces a remodeling effect in photodamaged skin
| Autor: | Sophie Deret, Carine Mounier, Florence Joly, Francesca Zolezzi, Bastien Gamboa, Corinne Ménigot, John T. Lear, Jérôme Aubert, Farzaneh Sidou, Pascale Reiniche, Johannes Voegel, Anthony A. Fryer, Paul Fogel |
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| Rok vydání: | 2017 |
| Předmět: |
Pathology
medicine.medical_specialty Epidermis (botany) business.industry medicine.medical_treatment Actinic keratosis Photodynamic therapy Dermatology medicine.disease Biochemistry Extracellular matrix Lesion Gene expression profiling 030207 dermatology & venereal diseases 03 medical and health sciences 0302 clinical medicine Real-time polymerase chain reaction 030220 oncology & carcinogenesis Gene expression medicine medicine.symptom business Molecular Biology |
| Zdroj: | Journal of dermatological science. |
| ISSN: | 1873-569X |
| Popis: | Background Actinic keratoses (AK) are proliferations of neoplastic keratinocytes in the epidermis resulting from cumulative exposure to ultraviolet radiation (UVR), which are liable to transform into squamous cell carcinoma (SCC). Organ Transplant Recipients (OTR) have an increased risk of developing SCC as a consequence of long-term immunosuppressive therapy. The aim of this study was to determine the molecular signature of AKs from OTR prior to treatment with methyl aminolevulinate-photodynamic therapy (MAL-PDT), and to assess what impact the treatment has on promoting remodeling of the photo-damaged skin. Methods Seven patients were enrolled on a clinical trial to assess the effect of MAL-PDT with biopsies taken at screening prior to the first treatment session (week 1), and six weeks after completion of final treatment (week 18). Whole-genome gene expression analysis was carried out on skin biopsies isolated from an AK lesion, an area surrounding the lesion, and a non-sun exposed region of the body. Quantitative PCR was utilized to confirm the differential expression of key genes. Results MAL-PDT treatment corrected abnormal proliferation-related gene profiles, corrected aberrantly expressed cancer-associated genes and induced expression of dermal extracellular matrix genes in photo-exposed skin. Conclusion The efficacy of the MAL-PDT on AK lesions was confirmed at whole-genome gene expression level. A transcriptional signature of remodeling, identified through assessing the effect of MAL-PDT on photodamaged skin, supports the use of MAL-PDT for treating photodamaged skin and field cancerized areas. |
| Databáze: | OpenAIRE |
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