Osteopontin expression and relation to disease severity in human asthma
| Autor: | Themis Alissafi, Vily Panoutsakopoulou, Stylianos Vittorakis, Georgina Xanthou, Mina Gaga, Apostolos Bossios, Erasmia Economidou, Eleftherios Zervas, H. Gogos, Jan Lötvall, Maria Semitekolou, Konstantinos Samitas |
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| Rok vydání: | 2010 |
| Předmět: |
Adult
Male Pulmonary and Respiratory Medicine Pathology medicine.medical_specialty Allergy Bronchi Inflammation Severity of Illness Index Basement Membrane Allergic inflammation stomatognathic system Bronchoscopy medicine Humans Mast Cells Osteopontin Myofibroblasts Aged Basement membrane medicine.diagnostic_test biology business.industry Respiratory disease Middle Aged respiratory system medicine.disease Asthma Up-Regulation respiratory tract diseases Cross-Sectional Studies Bronchoalveolar lavage medicine.anatomical_structure Immunology Reticular connective tissue biology.protein Airway Remodeling Female Goblet Cells medicine.symptom business Bronchoalveolar Lavage Fluid |
| Zdroj: | European Respiratory Journal. 37:331-341 |
| ISSN: | 1399-3003 0903-1936 |
| DOI: | 10.1183/09031936.00017810 |
| Popis: | Recent studies have associated osteopontin (OPN) with allergic inflammation; however, its role in human asthma remains unclear. The aim of this study was to measure OPN levels in the serum, bronchoalveolar lavage fluid (BALF) and bronchial tissue of healthy controls and asthmatics, identify cellular sources of OPN and examine possible correlations between OPN expression, disease severity and airway remodelling. Serum samples were obtained from 35 mild-to-moderate asthmatics, 19 severe asthmatics and 17 healthy controls in the steady state and in cases of exacerbation. Of these subjects, 29 asthmatics and nine controls underwent bronchoscopy with endobronchial biopsy and BALF collection. OPN expression was determined by ELISA and immunohistochemistry/immunofluorescence. Reticular basement membrane thickness and goblet cell hyperplasia were also determined. Serum and BALF OPN levels were significantly increased in all asthmatics in the steady state, whereas serum levels decreased during exacerbations. OPN was upregulated in the bronchial tissue of all patients, and expressed by epithelial, airway and vascular smooth muscle cells, myofibroblasts, T-lymphocytes and mast cells. OPN expression correlated with reticular basement membrane thickness and was more prominent in subepithelial inflammatory cells in severe compared to mild-to-moderate asthma. OPN expression is upregulated in human asthma and associated with remodelling changes, and its subepithelial expression correlates with disease severity. |
| Databáze: | OpenAIRE |
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