Cryopreservation of dog platelets with dimethyl sulfoxide: Therapeutic effectiveness of cryopreserved platelets in the treatment of thrombocytopenic dogs, and the effect of platelet storage at −80 °C
| Autor: | A.J. Melaragno, C. R. Valeri, H. Feingold, J J Vecchione |
|---|---|
| Rok vydání: | 1986 |
| Předmět: |
Blood Platelets
Pathology medicine.medical_specialty Therapeutic effectiveness Platelet Transfusion General Biochemistry Genetics and Molecular Biology Cryopreservation Blood cell Andrology chemistry.chemical_compound Dogs In vivo Freezing medicine Animals Blood Transfusion Dimethyl Sulfoxide Platelet biology Dimethyl sulfoxide Fissipedia General Medicine Platelet storage biology.organism_classification Thrombocytopenia medicine.anatomical_structure chemistry Blood Preservation General Agricultural and Biological Sciences |
| Zdroj: | Cryobiology. 23:387-394 |
| ISSN: | 0011-2240 |
| DOI: | 10.1016/0011-2240(86)90023-4 |
| Popis: | Dog platelets were frozen with 6% dimethyl sulfoxide at 2-3 degrees C per minute in a -80 degrees C mechanical freezer. The frozen platelets were stored at -80 degrees C for as long as 39 months. After storage at -80 degrees C for less than 1 year, platelet in vitro freeze-thaw-wash recovery values were 70%, and in vivo survival values 1 to 2 hr after transfusion were 40% those of fresh platelets. After 2 years or longer storage, in vitro freeze-thaw-wash recovery values were 60%, and in vivo survival values 1 to 2 hr after transfusion were 20% those of fresh platelets. These results indicate that significant deterioration of the dog platelets occurred between the first and second year of storage at -80 degrees C. Platelets that were stored frozen at -80 degrees C for less than 1 year and washed before transfusion into lethally irradiated thrombocytopenic dogs were hemostatically effective. |
| Databáze: | OpenAIRE |
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