ZNF703 promotes tumor cell proliferation and invasion and predicts poor prognosis in patients with colorectal cancer
| Autor: | Jide Wang, Yadong Wang, Ya-Li Zhang, Gongli Yang, Li-Hong Bi, Feng Ma, Cuiping Liu, Yingying Zhao, Yang Bai, Mengnan Zhang |
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| Rok vydání: | 2014 |
| Předmět: |
Adult
Male Cancer Research Pathology medicine.medical_specialty Colorectal cancer Biology medicine.disease_cause Cell Line Tumor medicine Humans RNA Small Interfering Lymph node Aged Aged 80 and over Gene knockdown Oncogene General Medicine Middle Aged Cell cycle Prognosis medicine.disease Survival Analysis Molecular medicine digestive system diseases Up-Regulation medicine.anatomical_structure Oncology Gene Knockdown Techniques Cancer research Immunohistochemistry Female Caco-2 Cells Carrier Proteins Colorectal Neoplasms Carcinogenesis HT29 Cells |
| Zdroj: | Oncology Reports. 32:1071-1077 |
| ISSN: | 1791-2431 1021-335X |
| DOI: | 10.3892/or.2014.3313 |
| Popis: | Zinc finger protein 703 (ZNF703), identified as an oncogene in luminal B breast cancer, is a member of the NET/NlZ family of zinc finger transcription factors. However, the role of ZNF703 in colorectal cancer (CRC) is unknown. We investigated the expression of ZNF703 in paired tumor and corresponding normal tissues using reverse transcriptase-polymerase chain reaction (RT-PCR) and western blot analysis. Immunohistochemistry (IHC) was applied on paraffin-embedded specimens, including 138 CRC tissues, 58 matched normal tissues and 30 paired metastatic lymph node samples. Levels of mRNA (72.72%, 16/22) and ZNF703 protein expression (65.38%, 17/26, respectively) were upregulated in CRC tissues. IHC staining revealed higher expression of ZNF703 in the CRC tissues (68/138, 49.3%) compared with that in the adjacent normal mucosal tissues (4/58, 6.9%) (p |
| Databáze: | OpenAIRE |
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