Stromal fibroblasts synthesize collagenase and stromelysin during long-term tissue remodeling
| Autor: | M.J. Tessier, C. Cintron, Claire L. Kublin, M T Girard, M. Matsubara, M E Fini |
|---|---|
| Rok vydání: | 1993 |
| Předmět: |
Matrix Metalloproteinase 3
Time Factors Stromal cell Connective tissue Biology Cornea medicine Animals Secretion Collagenases Fibroblast Wound Healing Metalloproteinase Metalloendopeptidases Cell Biology Fibroblasts Cell biology medicine.anatomical_structure Biochemistry Collagenase Rabbits Stromal Cells Wound healing Corneal Injuries Peptide Hydrolases medicine.drug |
| Zdroj: | Journal of Cell Science. 104:1001-1011 |
| ISSN: | 1477-9137 0021-9533 |
| Popis: | The process of connective tissue remodeling is an important mechanism contributing to tissue morphogenesis in development and homeostasis. Although it has long been known that remodeling tissues actively mediate collagenolysis, little is understood about the molecular mechanisms controlling this cell-regulated process. In this study, we examined the biosynthesis of collagenase and the related metalloproteinase, stromelysin, during remodeling of repair tissue deposited after mechanical injury to the rabbit cornea. Neither enzyme was synthesized by uninjured corneas; however, synthesis and secretion was detectable within one day after injury. Collagenase accumulated in its latent form while stromelysin appeared to be partially activated. Enzymes were synthesized by cells having a fibroblast phenotype. These cells were found within the stroma. New synthesis was correlated with accumulation of enzyme-specific mRNA. Highest levels of enzyme synthesis were observed in the repair tissue. However, stromal cells outside of the repairing area also synthesized both enzymes. The level of synthesis decreased in a gradient radiating from the repair tissue. Total synthetic levels in a given area of cornea were dependent on both the number of cells expressing enzyme and the rate of enzyme synthesis. Synthesis of collagenase was detected in repair tissue as long as nine months after injury. Our findings provide direct support for the hypothesis that new collagenase synthesis by cells in repair tissue is the first step in collagen degradation during long-term tissue remodeling. |
| Databáze: | OpenAIRE |
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