Type I collagen hydrogels as a delivery matrix for royal jelly derived extracellular vesicles
| Autor: | Orlando J Ramírez, Pamina Contreras-Kallens, Christina M.A.P. Schuh, Nelson P. Barrera, Simon Alvarez, Sebastian Aguayo |
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| Rok vydání: | 2020 |
| Předmět: |
food.ingredient
extracellular vesicle delivery Wound healing regenerative medicine Pharmaceutical Science RM1-950 02 engineering and technology Matrix (biology) 030226 pharmacology & pharmacy Extracellular vesicles Regenerative medicine Collagen Type I Extracellular Vesicles 03 medical and health sciences Drug Delivery Systems 0302 clinical medicine food Cell Movement Royal jelly Humans Dose-Response Relationship Drug Chemistry Fatty Acids Hydrogels General Medicine Fibroblasts 021001 nanoscience & nanotechnology Cell biology drug delivery Drug delivery Self-healing hydrogels Therapeutics. Pharmacology Apis mellifera 0210 nano-technology Type I collagen Research Article |
| Zdroj: | Drug Delivery article-version (VoR) Version of Record Drug Delivery, Vol 27, Iss 1, Pp 1308-1318 (2020) |
| ISSN: | 1521-0464 1071-7544 |
| DOI: | 10.1080/10717544.2020.1818880 |
| Popis: | Throughout the last decade, extracellular vesicles (EVs) have become increasingly popular in several areas of regenerative medicine. Recently, Apis mellifera royal jelly EVs (RJ EVs) were shown to display favorable wound healing properties such as stimulation of mesenchymal stem cell migration and inhibition of staphylococcal biofilms. However, the sustained and effective local delivery of EVs in non-systemic approaches – such as patches for chronic cutaneous wounds – remains an important challenge for the development of novel EV-based wound healing therapies. Therefore, the present study aimed to assess the suitability of type I collagen -a well-established biomaterial for wound healing – as a continuous delivery matrix. RJ EVs were integrated into collagen gels at different concentrations, where gels containing 2 mg/ml collagen were found to display the most stable release kinetics. Functionality of released RJ EVs was confirmed by assessing fibroblast EV uptake and migration in a wound healing assay. We could demonstrate reliable EV uptake into fibroblasts with a sustained pro-migratory effect for up to 7 d. Integrating fibroblasts into the RJ EV-containing collagen gel increased the contractile capacity of these cells, confirming availability of RJ EVs to fibroblasts within the collagen gel. Furthermore, EVs released from collagen gels were found to inhibit Staphylococcus aureus ATCC 29213 biofilm formation. Overall, our results suggest that type I collagen could be utilized as a reliable, reproducible release system to deliver functional RJ EVs for wound healing therapies. |
| Databáze: | OpenAIRE |
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