GPR56, a Novel Secretin-like Human G-Protein-Coupled Receptor Gene
| Autor: | Grant R. Sutherland, Nancy A. Jenkins, Karen Darby, Joanna Crawford, Debra J. Gilbert, Helen J. Eyre, Neal G. Copeland, Herbert Herzog, Marjorie Liu, Rachel M. C. Parker |
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| Rok vydání: | 1999 |
| Předmět: |
Molecular Sequence Data
Hypothalamus Thyroid Gland Receptors Cell Surface In situ hybridization Biology Models Biological G Protein-Coupled Receptor Gene Receptors G-Protein-Coupled Mice Secretin Gene mapping GTP-Binding Proteins Complementary DNA Gene expression Genetics medicine Animals Humans Tissue Distribution Amino Acid Sequence Gene Crosses Genetic In Situ Hybridization In Situ Hybridization Fluorescence Metaphase DNA Primers Gene Library Base Sequence medicine.diagnostic_test cDNA library Myocardium Chromosome Mapping Exons Blotting Northern Molecular biology Introns Rats Mice Inbred C57BL Chromosomes Human Pair 16 Fluorescence in situ hybridization |
| Zdroj: | Genomics. 55:296-305 |
| ISSN: | 0888-7543 |
| DOI: | 10.1006/geno.1998.5644 |
| Popis: | A novel gene product, GPR56, with homology to the seven transmembrane-domain receptor superfamily, has been cloned by PCR amplification using degenerate oligonucleotide primers and subsequent screening of a human heart cDNA library. The isolated 2.8-kb cDNA clone encodes a protein of 693 amino acids that shows highest identity (32%) to HE6, a member of a subclass of the class B secretin-like G-protein-coupled receptors. Northern analysis of various human tissues revealed a wide distribution of the transcript with highest levels found in thyroid gland, brain, and heart. In situ hybridization analysis of human thyroid gland as well as rat heart and brain tissue confirms these results and identifies the hippocampus and hypothalamic nuclei as brain areas with particularly high expression of GPR56 mRNA. The high level of mRNA expression, its wide distribution, and the mucin-like extracellular domain of the receptor protein suggest a possible role for this receptor in cell-cell interaction processes. The human gene for GPR56 has been isolated and its exon-intron structure determined. The total length of the human GPR56 gene is approximately 15 kb, and it consists of 13 exons. Fluorescence in situ hybridization, PCR analysis of somatic cell hybrids, and interspecific mouse backcross mapping have localized the genes to human chromosome 16q13 and mouse chromosome 8. |
| Databáze: | OpenAIRE |
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