Rhodococcus bacteria as a promising source of oils from olive mill wastes
| Autor: | Héctor M. Alvarez, Mariana P. Lanfranconi, O. Marisa Herrero, María S. Villalba |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Physiology Bioconversion 030106 microbiology ENGINEERED RHODOCOCCUS Industrial Waste INGENIERÍAS Y TECNOLOGÍAS Hexadecane Applied Microbiology and Biotechnology Actinobacteria Biotecnología Industrial 03 medical and health sciences chemistry.chemical_compound OLIVE MILL WASTES Bacterial Proteins Olea RNA Ribosomal 16S Rhodococcus Biomass Food science LIPID PRODUCTION Cloning Molecular Olive Oil Biotransformation Phylogeny Triglycerides chemistry.chemical_classification BIOCONVERSION biology Bioproductos Biomateriales Bioplásticos Biocombustibles Bioderivados etc Fatty Acids Chemical oxygen demand Fatty acid Gene Expression Regulation Bacterial General Medicine biology.organism_classification Lipids Culture Media chemistry Genes Bacterial Genetic Engineering Bacteria Biotechnology Olive oil |
| DOI: | 10.1007/s11274-018-2499-3 |
| Popis: | The accumulation of triacylglycerols (TAG) is a common feature among actinobacteria belonging to Rhodococcus genus. Some rhodococcal species are able to produce significant amounts of those lipids from different single substrates, such as glucose, gluconate or hexadecane. In this study we analyzed the ability of different species to produce lipids from olive oil mill wastes (OMW), and the possibility to enhance lipid production by genetic engineering. OMW base medium prepared from alperujo, which exhibited high values of chemical oxygen demand (127,000 mg/l) and C/N ratio (508), supported good growth and TAG production by some rhodococci. R. opacus, R. wratislaviensis and R. jostii were more efficient at producing cell biomass (2.2–2.7 g/l) and lipids (77–83% of CDW, 1.8–2.2 g/l) from OMW than R. fascians, R. erythropolis and R. equi (1.1–1.6 g/l of cell biomass and 7.1–14.0% of CDW, 0.1–0.2 g/l of lipids). Overexpression of a gene coding for a fatty acid importer in R. jostii RHA1 promoted an increase of 2.2 fold of cellular biomass value with a concomitant increase in lipids production during cultivation of cells in OMW. This study demonstrates that the bioconversion of OMW to microbial lipids is feasible using more robust rhodococal strains. The efficiency of this bioconversion can be significantly enhanced by engineering strategies. Fil: Herrero, Ornella Marisa. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de la Patagonia "San Juan Bosco"; Argentina Fil: Villalba, María Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de la Patagonia "San Juan Bosco"; Argentina Fil: Lanfranconi, Mariana Patricia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de la Patagonia "San Juan Bosco"; Argentina Fil: Alvarez, Hector Manuel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de la Patagonia "San Juan Bosco"; Argentina |
| Databáze: | OpenAIRE |
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