Identification of human complement factor H as a chemotactic protein for monocytes
| Autor: | Karim Nabil, Bertrand Rihn, Jean Ripoche, Nadine Martinet, Yves Martinet, Jean-Michel Vignaud, Marie-Claude Jaurand |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 1997 |
| Předmět: |
Mesothelioma
Chemotactic Factors CD46 Monocyte Thrombin Cell Biology Complement receptor Complement factor I Biology Biochemistry Molecular biology Monocytes Complement system Pleural Effusion Chemotaxis Leukocyte medicine.anatomical_structure Factor H Complement Factor H Alternative complement pathway medicine Humans Amino Acid Sequence Molecular Biology Decay-accelerating factor Research Article |
| Popis: | We used chromatographic separation to purify to homogeneity a monomeric monocyte chemotactic protein of 150 kDa contained in mesothelioma pleural effusions. It was identified by N-terminal amino acid sequencing and immunoblotting as complement factor H, an inhibitor of the alternative complement pathway. Specific antibodies against factor H inhibited the monocyte chemotactic activity of the purified protein, which was most active at 10 nM. Factor H is a restrictive factor of alternative complement pathway activation. The new chemotactic function assigned to factor H in recruiting monocytes to the mesothelioma site might contribute to malignant cell phagocytosis via the iC3b/complement receptor type 3 pathway. These functions link the humoral and cellular immune systems. |
| Databáze: | OpenAIRE |
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