Massive Gene Transfer and Extensive RNA Editing of a Symbiotic Dinoflagellate Plastid Genome
| Autor: | Senjie Lin, Ryo Koyanagi, Nori Satoh, Hiroki Goto, Makiko Tanaka, Takeshi Takeuchi, Takeshi Kawashima, Kanako Hisata, Chuya Shinzato, Eiichi Shoguchi, Manabu Fujie, Sutada Mungpakdee |
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| Rok vydání: | 2014 |
| Předmět: |
Models
Molecular RNA editing Nuclear gene Protein Conformation Symbiodinium minutum Genes Protozoan Genome Plastid Molecular Sequence Data Protozoan Proteins Biology dinoflagellate Minicircle Genome DNA sequencing chemistry.chemical_compound Gene duplication Genetics Amino Acid Sequence Plastids Gene Ecology Evolution Behavior and Systematics minicircles Base Sequence light-harvesting complex proteins hydropathy Biological Evolution plastid-associated genes chemistry Dinoflagellida RNA Protozoan DNA Research Article |
| Zdroj: | Genome Biology and Evolution |
| ISSN: | 1759-6653 |
| Popis: | Genome sequencing of Symbiodinium minutum revealed that 95 of 109 plastid-associated genes have been transferred to the nuclear genome and subsequently expanded by gene duplication. Only 14 genes remain in plastids and occur as DNA minicircles. Each minicircle (1.8-3.3 kb) contains one gene and a conserved noncoding region containing putative promoters and RNA-binding sites. Nine types of RNA editing, including a novel G/U type, were discovered in minicircle transcripts but not in genes transferred to the nucleus. In contrast to DNA editing sites in dinoflagellate mitochondria, which tend to be highly conserved across all taxa, editing sites employed in DNA minicircles are highly variable from species to species. Editing is crucial for core photosystem protein function. It restores evolutionarily conserved amino acids and increases peptidyl hydropathy. It also increases protein plasticity necessary to initiate photosystem complex assembly. |
| Databáze: | OpenAIRE |
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