Perspectives for Consideration in the Development of Microbial Cell Reference Materials
| Autor: | Joseph Russell Carmical, Emma Allen-Vercoe, Mitchell H. Gail, Samuel P. Forry, Rashmi Sinha |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Ideal (set theory) Bacteria Epidemiology Computer science Computational Biology Sequence Analysis DNA Reference Standards Article Characterization (materials science) 03 medical and health sciences 030104 developmental biology 0302 clinical medicine Oncology Metagenomics 030220 oncology & carcinogenesis Benchmark (computing) Humans Metagenome Biochemical engineering Biochemical markers |
| Zdroj: | Cancer Epidemiol Biomarkers Prev |
| Popis: | Microbiome measurement and analyses benefit greatly from incorporation of reference materials as controls. However, there are many points to consider in defining an ideal whole-cell reference material standard. Such a standard would embody all the diversity and measurement challenges present in real samples, would be completely characterized to provide “ground truth” data, and would be inexpensive and widely available. This ideal is, unfortunately, not readily attainable because of the diverse nature of different sequencing projects. Some applications may benefit most from highly complex reference materials, while others will value characterization or low expense more highly. The selection of appropriate microbial whole-cell reference materials to benchmark and validate microbial measurements should be considered carefully and may vary among specific applications. In this article, we describe a perspective on the development of whole-cell microbial reference materials for use in metagenomics analyses. |
| Databáze: | OpenAIRE |
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