Seeing the unseen: Imaging rotation in cells with designer anisotropic particles
| Autor: | Yanqi Yu, Yuan Gao, Yan Yu, Lucero Sanchez |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
Physics
Fluorescence-lifetime imaging microscopy Cellular functions General Physics and Astronomy Nanotechnology 02 engineering and technology Cell Biology 010402 general chemistry 021001 nanoscience & nanotechnology Tracking (particle physics) 01 natural sciences Cell function Article 0104 chemical sciences Structural Biology Single-particle tracking General Materials Science Anisotropic particles 0210 nano-technology Rotational dynamics Biological system Rotation (mathematics) |
| Popis: | Cellular functions are enabled by cascades of transient biological events. Imaging and tracking the dynamics of these events have proven to be a powerful means of understanding the principles of cellular processes. These studies have typically focused on translational dynamics. By contrast, investigations of rotational dynamics have been scarce, despite emerging evidence that rotational dynamics are an inherent feature of many cellular processes and may also provide valuable clues to understanding those cell functions. Such studies have been impeded by the limited availability of suitable rotational imaging probes. This has recently changed thanks to the advances in the development of anisotropic particles for rotational imaging. In this review, we will summarize current techniques for imaging rotation using particle probes that are anisotropic in shape or optical properties. We will highlight two studies that demonstrate how these techniques can be applied to explore important facets of cellular functions. |
| Databáze: | OpenAIRE |
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