Simultaneous PCR detection of Haemophilus ducreyi, Treponema pallidum, and herpes simplex virus types 1 and 2 from genital ulcers
| Autor: | K A Orle, D H Martin, B A Body, Judith B. Weiss, C A Gates |
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| Rok vydání: | 1996 |
| Předmět: |
DNA
Bacterial Male Microbiology (medical) Sexually transmitted disease Herpesvirus 2 Human Molecular Sequence Data Herpesvirus 1 Human urologic and male genital diseases medicine.disease_cause Polymerase Chain Reaction Sensitivity and Specificity Virus law.invention Microbiology Chancroid Haemophilus ducreyi law Virology Alphaherpesvirinae Multiplex polymerase chain reaction medicine Humans Syphilis Treponema pallidum Ulcer Polymerase chain reaction DNA Primers Bacteriological Techniques Herpes Genitalis Treponema Base Sequence biology biology.organism_classification Herpes simplex virus DNA Viral Colorimetry Genital Diseases Male DNA Probes Research Article |
| Zdroj: | Journal of Clinical Microbiology. 34:49-54 |
| ISSN: | 1098-660X 0095-1137 |
| DOI: | 10.1128/jcm.34.1.49-54.1996 |
| Popis: | A multiplex PCR (M-PCR) assay with colorimetric detection was devised for the simultaneous amplification of DNA targets from Haemophilus ducreyi, Treponema pallidum, and herpes simplex virus (HSV) types 1 and 2. By using target-specific oligonucleotides in a microwell format, 298 genital ulcer swab specimens collected in New Orleans during three intervals from 1992 through 1994 were evaluated. The results of the M-PCR assay were compared with the results of dark-field microscopy and H. ducreyi culture on two different culture media. HSV culture results were available for 99 specimens collected during the third interval. Confirmatory PCR assays targeting different gene sequences for each of the three organisms were used to validate the M-PCR results. Specimens were resolved as positive for the determination of sensitivity if the reference diagnostic test was positive or if the results of both the M-PCR and the confirmatory PCR were positive. The resolved sensitivities of M-PCR for HSV, H. ducreyi, and T. pallidum were 100, 98.4, and 91%, respectively. The resolved sensitivities of HSV culture, H. ducreyi culture, and dark-field microscopy were 71.8, 74.2, and 81%, respectively. These results indicate that the M-PCR assay is more sensitive than standard diagnostic tests for the detection of HSV, H. ducreyi, and T. pallidum from genital ulcers. |
| Databáze: | OpenAIRE |
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