H5N1 strain-specific Hemagglutinin CD4+ T cell epitopes restricted by HLA DR4
| Autor: | Eddie A. James, William W. Kwok, Laurie Huston, Betty B. Yue, Venus Tan, Gerald T. Nepom, Junbao Yang, John A. Gebe |
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| Rok vydání: | 2009 |
| Předmět: |
CD4-Positive T-Lymphocytes
Naive T cell animal diseases Epitopes T-Lymphocyte Hemagglutinin (influenza) Hemagglutinin Glycoproteins Influenza Virus Mice Transgenic Human leukocyte antigen Article Epitope Mice Immune system Immunity HLA-DR4 Antigen Animals Humans Cells Cultured Influenza A Virus H5N1 Subtype General Veterinary General Immunology and Microbiology biology Public Health Environmental and Occupational Health Virology Vaccination Infectious Diseases Epitope mapping Immunology biology.protein Molecular Medicine Epitope Mapping |
| Zdroj: | Vaccine. 27:3862-3869 |
| ISSN: | 0264-410X |
| DOI: | 10.1016/j.vaccine.2009.04.019 |
| Popis: | CD4+ T cells play a pivotal role in the viral immunity, and as such identification of unique strain-specific HLA class II restricted epitopes is essential for monitoring cellular strain-specific viral immunity. Using Tetramer-Guided Epitope Mapping technique, we identified HLA-DR0401 restricted HA epitopes that are strain-specific to H5N1 virion. Two immunodominant epitopes H5HA441–460 and H5HA57–76 were identified from in vitro stimulated human PBMC. Both epitopes elicit strong cellular immune responses when HLA-DR0401 transgenic mice are immunized with H5N1 subvirion indicating in vivo naturally processed immunodominant epitopes. The H5HA57–76 epitope is unique for the H5N1 strain but conserved among all H5N1 clades recommended for vaccine development by World Health Organization. The unique H5HA57–76 response was uncommon in unexposed individuals and only observed in the naive T cell subset. Thus, H5N1 strain-specific H5HA57–76 immunogenic epitope represents a unique marker for monitoring the efficacy of vaccination or as a candidate vaccine peptide. |
| Databáze: | OpenAIRE |
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