Alpha-2-macroglobulin is a modulator of prophenoloxidase system in pacific white shrimp Litopenaeus vannamai
Autor: | Tipachai Vatanavicharn, Vichien Rimphanitchayakit, Sirikwan Ponprateep, Anchalee Tassanakajon, Chu Fang Lo |
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Rok vydání: | 2016 |
Předmět: |
0301 basic medicine
animal structures Phagocytosis Litopenaeus Gene Expression Aquatic Science Microbiology Arthropod Proteins alpha-2-Macroglobulin 03 medical and health sciences Immune system Penaeidae medicine Environmental Chemistry Animals alpha-Macroglobulins Enzyme Precursors biology Vibrio parahaemolyticus General Medicine Prophenoloxidase biology.organism_classification Trypsin Immunity Innate Recombinant Proteins Shrimp 030104 developmental biology Gene Knockdown Techniques biology.protein Serine Proteases Catechol Oxidase medicine.drug |
Zdroj: | Fishshellfish immunology. 62 |
ISSN: | 1095-9947 |
Popis: | The shrimp multifunctional protein alpha-2-macroglobulin (A2M) is abundantly expressed in plasma, highly up-regulated upon microbial infection and involved in several immune pathways such as blood clotting system, phagocytosis and melanization. Herein, the function of LvA2M from Litopenaeus vannamei on the prophenoloxidase (proPO) system is reported. The recombinant (r)LvA2M produced strongly and specifically inhibited trypsin and the PO activity in shrimp plasma in a dose-dependent manner. Silencing of LvA2M led to an increase in the PO activity in shrimp plasma although the expression of proPO-associated genes, proPO-activating enzyme (PPAE) and prophenoloxidase (proPO) but not the proPO-activating factor (PPAF) was down-regulated. In Vibrio parahaemolyticus AHPND-infected shrimp, the LvA2M activity was suppressed in an early phase of infection while the PO activity was increased. Thus, the proPO-activating system was regulated by the LvA2M. |
Databáze: | OpenAIRE |
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