Localization and Changes of Tyrosine Phosphorylated Proteins and ß Actin in Epididymis of Rats Treated with Valproic Acid
| Autor: | Supatcharee Arun, Amnart Chaichun, Apichakarn Sampannang, Saranya Tongpan, Sitthichai Iamsaard, Chanwit Maneenin, Jaturon Burawat, Wannisa Sukhorum, Tarinee Sawatpanich |
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| Rok vydání: | 2018 |
| Předmět: |
Beta actin
Valproic Acid 030219 obstetrics & reproductive medicine Tyrosine phosphorylated proteins Chemistry Epididymal fluid 030232 urology & nephrology Epididymis Molecular biology ácido valproico Rats 03 medical and health sciences 0302 clinical medicine medicine.anatomical_structure Localization medicine Phosphorylation Immunohistochemistry lipids (amino acids peptides and proteins) Anatomy Tyrosine Phosphorylated proteins Actin medicine.drug |
| Zdroj: | International Journal of Morphology v.36 n.3 2018 SciELO Chile CONICYT Chile instacron:CONICYT |
| ISSN: | 0717-9502 |
| DOI: | 10.4067/s0717-95022018000300835 |
| Popis: | SUMMARY: Tyrosine phosphorylated proteins have been localized and identified in male reproductive tissues such as testis and capacitated/ acrosome reacted sperm except epididymis. The changes of such proteins are associated with decreased sperm quality of valproic acid treatment. This study aimed to investigate the presence and alterations of protein phosphorylation in epididymal epithelium and fluid of rats treated VPA. Sixteen adult male rats were divided into control and VPA-treated groups (n=8/ each). Treated rats were injected with VPA (500 mg/ kgBW, intraperitoneally) for 10 consecutive days. At the end of experiment, the monoclonal antiphosphotyrosine (clone 4G10) was used for immunohistochemistry to probe tyrosine phosphorylated proteins and also to examine the expression of such proteins using immuno-Western blotting in epididymal tissue and fluid. The result showed that positive reactivity of phosphorylated proteins was clearly observed in cytoplasmic principle cells, nuclei of apical & basal cells and sperm mass surrounded with epididymal fluids. The profiles of phosphorylated proteins in epididymal fluid were 182, 127, 80, 70, 57, 45, 34, and 31 kDas, respectively. Interestingly, VPA affected the changes of phosphorylated proteins and β actin in head, body, and tail epididymal fluids. We conclude that tyrosine phosphorylated proteins were detected in epididymal epithelium and fluid. The expressions of those proteins and actin were altered under VPA treating. |
| Databáze: | OpenAIRE |
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