CK1δ and CK1ε are components of human 40S subunit precursors required for cytoplasmic 40S maturation
| Autor: | Ivo Zemp, Christian Montellese, Emanuel Wyler, Ulrike Kutay, Sanjana Rao, Franziska Wandrey, Caroline Ashiono |
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| Rok vydání: | 2014 |
| Předmět: |
Gene isoform
Cytoplasm Casein Kinase 1 epsilon Protein subunit Ribosome biogenesis Biology 03 medical and health sciences 0302 clinical medicine Humans Eukaryotic Small Ribosomal Subunit Phosphorylation Protein Precursors Protein kinase A Nuclear export signal 030304 developmental biology Ribosome Subunits Small Eukaryotic 0303 health sciences Cell Biology Cell biology Protein Subunits Protein Transport HEK293 Cells Biochemistry RNA Ribosomal Casein Kinase Idelta Trans-Activators Casein kinase 1 Cell Adhesion Molecules Protein Processing Post-Translational 030217 neurology & neurosurgery Biogenesis HeLa Cells |
| Zdroj: | JOURNAL OF CELL SCIENCE |
| ISSN: | 1477-9137 |
| Popis: | Biogenesis of 40S pre-ribosomal subunits requires many trans-acting factors, among them several protein kinases. In this study, we show that the human casein kinase 1 (CK1) isoforms δ and e are required for cytoplasmic maturation steps of 40S subunit precursors. We show that both CK1δ and CK1e isoforms are components of pre-40S subunits, on which they phosphorylate the ribosome biogenesis factors ENP1/BYSL and LTV1. Inhibition or co-depletion of CK1δ and CK1e results in failure to recycle a series of trans-acting factors including ENP1/BYSL, LTV1, RRP12, DIM2/PNO1, RIO2 and NOB1 from pre-40S particles after nuclear export. Furthermore, co-depletion of CK1δ and CK1e leads to defects in 18S-E pre-rRNA processing. Together, these data demonstrate that CK1δ and CK1e play a decisive role in triggering late steps of pre-40S maturation that are required for acquisition of functionality of 40S ribosomal subunits in protein translation. |
| Databáze: | OpenAIRE |
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