Starch-enriched diet modulates the glucidic profile in the rat colonic mucosa
| Autor: | Daniele Tomassoni, Maria Gabriella Gabrielli |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Glycosylation Colon Glycoconjugate Medicine (miscellaneous) Sialidase Rats Sprague-Dawley 03 medical and health sciences chemistry.chemical_compound Intestinal mucosa Lectins Animals Fucosidase Intestinal Mucosa Fucose Glycoproteins chemistry.chemical_classification Nutrition and Dietetics Microvilli biology Mucin Mucins Lectin Starch N-Acetylneuraminic Acid Sialic acid Enterocytes 030104 developmental biology Carbohydrate Sequence chemistry Biochemistry biology.protein Female Indicators and Reagents Goblet Cells Diet Carbohydrate Loading Glycolipids Glycoprotein |
| Zdroj: | European Journal of Nutrition. 57:1109-1121 |
| ISSN: | 1436-6215 1436-6207 |
| Popis: | The protective function of the intestinal mucosa largely depends on carbohydrate moieties that as a part of glycoproteins and glycolipids form the epithelial glycocalyx or are secreted as mucins. Modifications of their expression can be induced by an altered intestinal microenvironment and have been associated with inflammatory disorders and colorectal cancer. Given the influence of dietary factors on the gut ecosystem, here we have investigated whether a long term feeding on a starch-rich diet can modulate the glucidic profile in the colonic mucosa of rats. Animals were divided into two groups and maintained for 9 months at different diets: one group was fed a standard diet, the second was fed a starch-enriched diet. Samples of colonic mucosa, divided in proximal and distal portions, were processed for microscopic analysis. Conventional stainings and lectin histochemistry were applied to identify acidic glycoconjugates and specific sugar residues in oligosaccharide chains, respectively. Some lectins were applied on adjacent sections after sialidase/fucosidase digestion, deacetylation, and oxidation to characterize either terminal dimers or sialic acid acetylation. An increase in sulfomucins was found to be associated with the starch-enriched diet that affected also the expression of several sugar residues as well as fucosylated and sialylated sequences in both proximal and distal colon. Although the mechanisms leading to such a modulation are at present unknown, either an altered intestinal microbiota or a dysregulation of glycosylation patterns might be responsible for the types and distribution of changes in the glucidic profile here observed. |
| Databáze: | OpenAIRE |
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