Translation of 33S mRNA, prepared from bovine thyroid glands, into thyroglobulin-like protein in a reticulocyte lysate cell-free system

Autor:	Wieland Gevers, Martin Pammenter, Andre J. Bester, Pieter van Jaarsveld
Rok vydání:	1977
Předmět:	endocrine system Reticulocytes endocrine system diseases Immunoprecipitation medicine.medical_treatment Biophysics Thyroid Gland Heterologous Biology Biochemistry Thyroglobulin chemistry.chemical_compound Reticulocyte medicine Protein biosynthesis Animals Trypsin RNA Messenger Sodium dodecyl sulfate Molecular Biology Polyacrylamide gel electrophoresis Cell-Free System Thyroid Cell Biology Molecular biology Precipitin Tests Peptide Fragments Molecular Weight medicine.anatomical_structure chemistry Protein Biosynthesis Cattle Rabbits
Zdroj:	Biochemical and biophysical research communications. 78(1)
ISSN:	0006-291X
Popis:	33S mRNA was isolated from bovine thyroid glands and translated in a heterologous cell-free amino acid incorporating system into a 12–19S protein judged to be thyroglobulin-like by immunoprecipitation, sodium dodecyl sulfate polyacrylamide gel electrophoresis and ion exchange chromatography.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e4fa62d57027d9e06f0358fd2d772250 https://pubmed.ncbi.nlm.nih.gov/907672 Zobrazit plný text záznamu