A chain-elongated oligophenylenevinylene electrolyte increases microbial membrane stability
| Autor: | Thomas Seviour, James C. S. Ho, Staffan Kjelleberg, Alex S. Moreland, Jamie Hinks, Bo Liedberg, Atul N. Parikh, Guillermo C. Bazan, Geraldine W. N. Chia, Cheng Zhou |
|---|---|
| Přispěvatelé: | School of Chemical and Biomedical Engineering, Interdisciplinary Graduate School (IGS), School of Materials Science and Engineering, Singapore Centre for Environmental Life Sciences and Engineering (SCELSE) |
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
Lipopolysaccharides
Materials science Vinyl Compounds Butanols Context (language use) 02 engineering and technology Microbial Sensitivity Tests Conjugated system 010402 general chemistry 01 natural sciences chemistry.chemical_compound Electrolytes Butanol Tolerance Cell Wall Escherichia coli General Materials Science Microscopy Confocal Mechanical Engineering Butanol Vesicle Chemical engineering [Engineering] Biological membrane 021001 nanoscience & nanotechnology 0104 chemical sciences Membrane chemistry Mechanics of Materials Biofuels Glycerophospholipid Biophysics lipids (amino acids peptides and proteins) 0210 nano-technology Bacterial outer membrane |
| Popis: | A novel conjugated oligoelectrolyte (COE) material, named S6, is designed to have a lipid-bilayer stabilizing topology afforded by an extended oligophenylenevinylene backbone. S6 intercalates biological membranes acting as a hydrophobic support for glycerophospholipid acyl chains. Indeed, Escherichia coli treated with S6 exhibits a twofold improvement in butanol tolerance, a relevant feature to achieve within the general context of modifying microorganisms used in biofuel production. Filamentous growth, a morphological stress response to butanol toxicity in E. coli, is observed in untreated cells after incubation with 0.9% butanol (v/v), but is mitigated by S6 treatment. Real-time fluorescence imaging using giant unilamellar vesicles reveals the extent to which S6 counters membrane instability. Moreover, S6 also reduces butanol-induced lipopolysaccharide release from the outer membrane to further maintain cell integrity. These findings highlight a deliberate effort in the molecular design of a chain-elongated COE to stabilize microbial membranes against environmental challenges. Ministry of Education (MOE) Nanyang Technological University C.Z. and G.W.N.C. contributed equally to this work. G.C.B. thanks the NTU start up grant M4081984.120. Work at SCELSE was supported by the Singapore Ministry of Education through grant M4360005. Work at UCSB was supported by the Institute for Collaborative Biotechnologies through grant W911NF-09-D-0001. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|