The Barrier-to-Autointegration Factor Is a Component of Functional Human Immunodeficiency Virus Type 1 Preintegration Complexes
| Autor: | Chou-Wen Lin, Alan Engelman |
|---|---|
| Rok vydání: | 2003 |
| Předmět: |
endocrine system
Immunoprecipitation Virus Integration Immunology Barrier-to-autointegration factor HIV Integrase Biology Microbiology DNA-binding protein Virology Complementary DNA Animals Humans HMGA1a Protein Nuclear protein Ku Autoantigen Gene Products vpr DNA Helicases Nuclear Proteins Antigens Nuclear vpr Gene Products Human Immunodeficiency Virus biochemical phenomena metabolism and nutrition Precipitin Tests Molecular biology HMGA1 Virus-Cell Interactions Integrase DNA-Binding Proteins Insect Science HIV-1 biology.protein Rabbits |
| Zdroj: | Journal of Virology. 77:5030-5036 |
| ISSN: | 1098-5514 0022-538X |
| DOI: | 10.1128/jvi.77.8.5030-5036.2003 |
| Popis: | Retroviral integration in vivo is mediated by preintegration complexes (PICs) derived from infectious virions. In addition to the integrase enzyme and cDNA substrate, PICs contain a variety of viral and host cell proteins. Whereas two different cell proteins, high-mobility group protein A1 (HMGA1) and the barrier-to-autointegration factor (BAF), were identified as integration cofactors based on activities in in vitro PIC assays, only HMGA1 was previously identified as a PIC component. By using antibodies against known viral and cellular PIC components, we demonstrate here functional coimmunoprecipitation of endogenous BAF protein with human immunodeficiency virus type 1 (HIV-1) PICs. Since integrase protein and integration activity were also coimmunoprecipitated by anti-BAF antibodies, we conclude that BAF is a component of HIV-1 PICs. These data are consistent with the model that BAF functions as an integration cofactor in vivo. |
| Databáze: | OpenAIRE |
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