In vitro activity of imipenem/relebactam against Gram-negative clinical isolates in two Spanish tertiary hospitals
| Autor: | Cristina García-Salguero, Esther Culebras, José Luis del Pozo, Marina Peñuelas, Francisco Javier Candel, Melania Iñigo, José Manuel Viñuela-Prieto |
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| Rok vydání: | 2021 |
| Předmět: |
Microbiology (medical)
Serial dilution Klebsiella pneumoniae Microbial Sensitivity Tests medicine.disease_cause beta-Lactamases Microbiology oxacillinase 48 Tertiary Care Centers Escherichia coli polycyclic compounds medicine Humans Imipenem+Relebactam Gram Pharmacology beta-lactamasa de espectro extendido biology Pseudomonas aeruginosa Brief Report Carbapenem-resistant Enterobacterales Broth microdilution General Medicine biochemical phenomena metabolism and nutrition bacterial infections and mycoses biology.organism_classification In vitro Imipenem-relebactam Extended-spectrum beta-lactamase Anti-Bacterial Agents Acinetobacter baumannii Enterobacteriaceae resistentes a los carbapenémicos Imipenem Klebsiella pneumoniae carbapenemase Azabicyclo Compounds |
| Zdroj: | Revista Española de Quimioterapia |
| ISSN: | 1988-9518 0214-3429 |
| DOI: | 10.37201/req/102.2021 |
| Popis: | Objetive. The aim of this study was to analyze the activity of the imipenem-relebactam combination (IMI/REL) against a collection of multidrug-resist Enterobacterales, Pseudomonas aeruginosa and Acinetobacter baumannii clinical isolates. Material and methods. The study was conducted in two tertiary hospitals in Spain and included 192 clinical isolates of these 3 genera (139 resistant and 53 susceptible to IMI). The MICs for IMI with and without REL (at a fixed concentration of 4 mg/L) were determined by a standard broth microdilution method according to international recommendations. Results. All IMI-susceptible E. coli strains were also susceptible to IMI/REL. Enterobacterales resistant to IMI due to the production of carbapenemases, the MIC50 and MIC90 decreased from 64/256 with IMI to 8/64 mg/L with IMI/REL. This high activity was principally detected among isolates with KPC enzymes. Enterobacterales with class B carbapenemases, P. aeruginosa carrying VIM carbapenemase and A. baumannii strains showed no changes on IMI MIC50 or MIC90 after adding REL. Among P. aeruginosa strains without carbapenemase the MIC for IMI/REL was reduced between 1 to 5 dilutions. Conclusions. IMI/REL showed high activity against the strains that carry Klebsiella pneumoniae carbapenemase (KPC) and against carbapenem-resistant P. aeruginosa unrelated to the VIM enzyme, mainly AmpC beta lactamase associated with impermeability. Against strains carrying oxacillinase 48 (OXA-48) associated with extended-spectrum beta-lactamase (ESBL), IMI/REL presented activity only slightly better than IMI and had no beneficial effect superior to IMI against A. baumannii. |
| Databáze: | OpenAIRE |
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