Immunohistochemical expression of P-glycoprotein and glutathione S-transferases in cerebral gliomas and response to chemotherapy
| Autor: | Knut Dietzmann, Sabine Diete, Elmar Kirches, M. Warich-Kirches, P. von Bossanyi |
|---|---|
| Rok vydání: | 1998 |
| Předmět: |
Pathology
medicine.medical_specialty medicine.medical_treatment Astrocytoma Pathology and Forensic Medicine Cellular and Molecular Neuroscience Glioma Antineoplastic Combined Chemotherapy Protocols medicine Humans ATP Binding Cassette Transporter Subfamily B Member 1 Karnofsky Performance Status Grading (tumors) P-glycoprotein Glutathione Transferase Teniposide Chemotherapy biology Brain Neoplasms medicine.disease Immunohistochemistry Isoenzymes medicine.anatomical_structure Glutathione S-transferase Nimustine Drug Resistance Neoplasm biology.protein Neurology (clinical) Antibody Glioblastoma Astrocyte |
| Zdroj: | Acta neuropathologica. 94(6) |
| ISSN: | 0001-6322 |
| Popis: | The expression of the drug resistance-related proteins glutathione S-transferases (GST) and P-glycoprotein (Pgp) was analyzed quantitatively in samples of 53 astrocytic gliomas (eight WHO grade 1, 11 WHO grade 2, 9 WHO grade 3 and 25 glioblastomas, WHO grade 4). Sections of these tumors were immunohistochemically stained with antibodies to Pgp (MDR1-gene product) and to GST subclasses alpha, mu and pi. Pgp expression was not detected in tumor cells of the majority of low-grade astrocytomas (69%) and the percentage of Pgp stained cells generally increased with tumor grade. However, 4 of the 34 malignant gliomas were negative. Many neoplastic cells of most tumors were dominantly stained for GST-pi. The other two subclasses were expressed in a less consistent fashion with no linear correlation to grading. Grade 2 astrocytomas exhibited the highest percentage of cells with GST expression. GST-alpha was absent in 9 tumors, GST-mu in 8 and GST-pi in 4. Four tumors showed no expression of any GST subclass or Pgp in neoplastic cells. Of 13 patients 5 with a more favorable clinical course after radiation and chemotherapy had a lower percentage of neoplastic cells immunostained for Pgp and the three GST subclasses than 8 patients with a worse clinical course. These results suggest a relationship between expression of drug resistance-related proteins in gliomas and response to chemotherapy with ACNU/VM26. |
| Databáze: | OpenAIRE |
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