Heterologous overexpression of a monotopic glucosyltransferase (MGS) induces fatty acid remodeling in Escherichia coli membranes

Autor: Åke Wieslander, Jonny Eriksson, Daniel O. Daley, Katarina Edwards, Ljubica Lindholm, Hansjörg Götzke, Andreas Barth, Candan Ariöz
Jazyk: angličtina
Předmět:
Models
Molecular
Monotopic membrane protein
Membrane lipids
Biophysics
cyclopropanation
medicine.disease_cause
Biochemistry
Intracellular membrane
Protein Structure
Secondary
Membrane Lipids
chemistry.chemical_compound
Escherichia coli
Membrane fluidity
medicine
Acholeplasma laidlawii
Lipid–protein interactions
Phosphatidylethanolamine
chemistry.chemical_classification
ATP synthase
biology
Phosphatidylethanolamines
Cyclopropanation
Cell Membrane
Fatty Acids
membrane fluidity
Biochemistry and Molecular Biology
Membrane Proteins
Fatty acid
intracellular membranes
Lipid–protein interaction
Kemi
Methyltransferases
Cell Biology
Fatty acid modeling
Membrane
chemistry
Membrane protein
Glucosyltransferases
Chemical Sciences
fatty acid modeling
biology.protein
monotopic membrane proteins
Biokemi och molekylärbiologi
Zdroj: Biochimica et Biophysica Acta (BBA) - Biomembranes. (7):1862-1870
ISSN: 0005-2736
DOI: 10.1016/j.bbamem.2014.04.001
Popis: The membrane protein monoglucosyldiacylglycerol synthase (MGS) from Acholeplasma laidlawii is responsible for the creation of intracellular membranes when overexpressed in Escherichia coli (E. coli). The present study investigates time dependent changes in composition and properties of E. coli membranes during 22h of MGS induction. The lipid/protein ratio increased by 38% in MGS-expressing cells compared to control cells. Time-dependent screening of lipids during this period indicated differences in fatty acid modeling. (1) Unsaturation levels remained constant for MGS cells (~62%) but significantly decreased in control cells (from 61% to 36%). (2) Cyclopropanated fatty acid content was lower in MGS producing cells while control cells had an increased cyclopropanation activity. Among all lipids, phosphatidylethanolamine (PE) was detected to be the most affected species in terms of cyclopropanation. Higher levels of unsaturation, lowered cyclopropanation levels and decreased transcription of the gene for cyclopropane fatty acid synthase (CFA) all indicate the tendency of the MGS protein to force E. coli membranes to alter its usual fatty acid composition.
Databáze: OpenAIRE
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