The rggC locus, with a frameshift mutation, is involved in oxidative stress response by Streptococcus thermophilus

Autor: Nathalie Leblond-Bourget, Brigitte Gintz, Frédéric Borges, Annabelle Fernandez, Bernard Decaris
Přispěvatelé: Laboratoire de génétique et microbiologie (LGM), Institut National de la Recherche Agronomique (INRA)-Université Henri Poincaré - Nancy 1 (UHP)
Jazyk: angličtina
Rok vydání: 2006
Předmět:
Streptococcus thermophilus
Transcription
Genetic
Mutant
MESH: Genome
Bacterial
Biochemistry
chemistry.chemical_compound
Menadione
MESH: Reverse Transcriptase Polymerase Chain Reaction
Transcriptional regulation
stress response AE Transcriptional regulator AE Rgg AE
ORFS
Frameshift Mutation
MESH: Bacterial Proteins
Genetics
0303 health sciences
MESH: Gene Expression Regulation
Bacterial
MESH: Oxidative Stress
biology
Reverse Transcriptase Polymerase Chain Reaction
MESH: Frameshift Mutation
Vitamin K 3
General Medicine
MESH: Vitamin K 3
[SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry
Molecular Biology/Biomolecules [q-bio.BM]
DNA-Binding Proteins
MESH: Trans-Activators
Genetic variability AE Frameshift mutation
Locus (genetics)
Microbiology
Frameshift mutation
MESH: Streptococcus thermophilus
03 medical and health sciences
Open Reading Frames
Bacterial Proteins
MESH: Polymorphism
Genetic
Molecular Biology
030304 developmental biology
Polymorphism
Genetic
030306 microbiology
MESH: Transcription
Genetic
[SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry
Molecular Biology/Molecular biology
Gene Expression Regulation
Bacterial
MESH: Open Reading Frames
biology.organism_classification
Molecular biology
[SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology
Open reading frame
Oxidative Stress
chemistry
Trans-Activators
Streptococcus thermophilus AE Oxidative
Genome
Bacterial
MESH: DNA-Binding Proteins
Zdroj: Archives of Microbiology
Archives of Microbiology, Springer Verlag, 2006, 186 (3), pp.161-169. ⟨10.1007/s00203-006-0130-8⟩
ISSN: 0302-8933
1432-072X
DOI: 10.1007/s00203-006-0130-8⟩
Popis: International audience; In Streptococcus thermophilus, the locus rggC contains a frameshift mutation and thus consists of two open reading frames (ORFs), rggC 1 and rggC 2 , which encode proteins exhibiting similarity with the Rgg transcriptional regulator family. In this work, mutants showing a partial deletion of rggC 1 and rggC 2 were constructed and their response to menadione, a superoxide-generating compound, was analysed. These mutants exhibited different behaviour to this oxidative stress compared with the wild-type strain. Analysis of this locus among 21 strains of S. thermophilus showed a polythymine tract length variability and a strain-dependant adenine residue could be found upstream of this repeat. This interstrain polymorphism supports evidence for the hypothesis that the rggC locus is phase variable.
Databáze: OpenAIRE
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