Protective effects of minocycline, doxycycline and tetracycline on seizure and lethality in a mice cocaine toxicity model
| Autor: | Hasan Avci, Aykut Yuksel, Aykut Uyanik, Bulent Erdur, Tarık Bektas, Atakan Yilmaz, Mert Ozen |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
Male
Minocycline mortality rate Pharmacology lethality Mice Random Allocation Bagg albino mouse Cocaine dose response Animals Anti-Bacterial Agents/*therapeutic use Cocaine/*toxicity Dose-Response Relationship Drug Doxycycline/*therapeutic use Drug Overdose/complications/*drug therapy/mortality Illicit Drugs/toxicity Mice Inbred BALB C Minocycline/*therapeutic use Seizures/chemically induced/*prevention & control Tetracycline/*therapeutic use Toxicity Tests Treatment Outcome animal drug determination ED50 comparative study Doxycycline drug effect General Medicine Anti-Bacterial Agents antiinfective agent Dose–response relationship priority journal Emergency Medicine Cocaine intoxication medicine.drug survival rate Tetracycline seizure animal experiment complication randomization Placebo Article acute cocaine intoxication toxicity testing Seizures medicine controlled study survival time mouse tetracycline nonhuman business.industry Illicit Drugs animal model Lethal dose toxicity drug intoxication medicine.disease mortality drug overdose placebo Drug Overdose business disease activity |
| Popis: | Introduction: Acute cocaine intoxication is one of the important causes of admission to emergency department, especially in western countries. We aimed to compare the efficacies of tetracycline, minocycline, doxycycline in the prevention of seizures and deaths in mice due to cocaine intoxication. Methods: In the study, a total of 120 balb-c male mice weighing 25–30 g were randomized into 4 groups as tetracycline 255 mg/kg, minocycline 170 mg/kg, doxycycline 157 mg/kg, 0.5 ml saline (placebo). The doses of tetracycline, minocycline and doxycycline are the calculated ED50 values. The mice in the groups received 93 mg/kg cocaine intraperitoneally 10 min after drug administration. The dose of cocaine is 50% of the lethal dose. After cocaine injection, all mice were observed for 30 min in terms of cocaine toxicity findings. Mortality rates, death times, seizure activities, and seizure onset times of the mice were clinically evaluated in an observational way. Results: There were significant differences among all the groups in terms of seizure and lethality (p < 0.001). The ratio of animals with seizures was significantly lower in the minocycline (73.3%), and doxycycline (73.3%) groups (all p = 0.040). The ratio of animals with lethality was significantly lower in the minocycline (23.3%) group compared with vehicle (p < 0.001). Conclusion: In our acute cocaine intoxication model, minocycline was effective in terms of lethality and preventing seizures, doxycycline was effective in preventing seizures. © 2019 Elsevier Inc. |
| Databáze: | OpenAIRE |
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