Dietary Guanidinoacetic acid modulates testicular histology and expression of c-Kit and STRA8 genes in roosters
| Autor: | Ali Nasirikhah, Mostafa Sadeghi, Hamid Deldar, Ali Reza Yousefi, Mahdi Zhandi, Malak Shakeri, Mahdi Ansari |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
Male
endocrine system congenital hereditary and neonatal diseases and abnormalities Glycine Rooster Testicle Biology Andrology 03 medical and health sciences Semen quality Basal (phylogenetics) Random Allocation 0302 clinical medicine Food Animals Gene expression Testis medicine Animals Small Animals Adaptor Proteins Signal Transducing 030219 obstetrics & reproductive medicine Equine 0402 animal and dairy science Broiler nutritional and metabolic diseases Histology 04 agricultural and veterinary sciences biology.organism_classification 040201 dairy & animal science Animal Feed Diet Semen Analysis Proto-Oncogene Proteins c-kit medicine.anatomical_structure Seminiferous tubule Gene Expression Regulation Animal Science and Zoology Animal Nutritional Physiological Phenomena Chickens |
| Zdroj: | Theriogenology. 130 |
| ISSN: | 1879-3231 1300-1450 |
| Popis: | Decline in semen quality is considered as a major contributing factor in age-related subfertility of broiler breeder flocks. This study was aimed to investigate the effect of dietary supplementation of Guanidinoacetic acid (GAA), as an alternative energy source along with antioxidant potential, on testicular histology and relative gene expression of some spermatogonial markers (c-Kit and STRA8) in aged roosters. Sixteen 24-week-old male broiler breeders were randomly allocated into four groups and fed a basal diet supplemented with increasing levels of GAA including 0 (GAA-0), 600 (GAA-600), 1200 (GAA-1200) or 1800 (GAA-1800) mg/kg diet/day for 26 successive weeks. At the end of the experiment, all the birds were killed and two ipsilateral testicle samples were taken to either quantify relative gene expression or do histology. Except for seminiferous tubules' diameter, testicular weight, and the number of blood vessels, dietary supplementation of GGA improved the epithelium thickness of seminiferous tubules, the number of spermatogonia and Leydig cells and the relative gene expression of c-Kit and STRA8 (P 0.01). Increasing levels of GAA cubically affected (P 0.01) the diameter of seminiferous tubules and their epithelium thickness as well as the number of spermatogonia. However, number of Leydig cells and relative expression of c-Kit were linearly, and relative expression of STRA8 was quadratically (P 0.01) enhanced in response to graded levels of GAA supplementation. Taking all parameters into account, daily supplementation of 1300-1450 mg of GAA/kg diet was estimated as an optimum dosage maximizing the evaluated traits. |
| Databáze: | OpenAIRE |
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