Multiple mitochondrial DNA deletions in an elderly human individual
| Autor: | Anthony W. Linnane, Ronald J. Maxwell, Alessandra Baumer, Chunfang Zhang, Phillip Nagley |
|---|---|
| Rok vydání: | 1992 |
| Předmět: |
Mitochondrial DNA
Base pair Mitochondrial disease Molecular Sequence Data Somatic gene mutation Biophysics Biology Biochemistry DNA Mitochondrial Polymerase Chain Reaction DNA sequencing law.invention Structural Biology law Genetics medicine Direct repeat Humans Molecular Biology Polymerase chain reaction Aged DNA deletion Base Sequence Multiple mitochondrial DNA deletions Muscles Myocardium Brain Cell Biology medicine.disease Molecular biology Ageing Female Primer (molecular biology) Chromosome Deletion Human |
| Zdroj: | FEBS letters. 297(1-2) |
| ISSN: | 0014-5793 |
| Popis: | We have used the polymerase chain reaction (PCR) to study deletions in the mitochondrial DNA (mtDNA) of an elderly human individual. An extended set of PCR primers has been utilised to identify 10 mitochondrial DNA deletions in a 69-year-old female subject with no known mitochondrial disease. The particular deletions visualised as PCR products depended on the primer pairs used, such that the more distantly separated PCR primers enabled visualisation of larger deletions. Some deletions were common to the heart, brain and skeletal muscle, whereas others were apparently specific to individual tissues. DNA sequencing analysis of PCR products showed that short direct repeat sequences (5 to 13 bp) flanked all deletion breakpoints; in most cases one copy of the repeat was deleted. It is proposed that the accumulation of such multiple deletions is a general phenomenon during the ageing process. |
| Databáze: | OpenAIRE |
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