Anti-stress and Adaptogenic Activity of l-Arginine Supplementation

Autor: Surinder K. Grover, Asheesh Gupta, Ratan Kumar, H. M. Divekar, Vanita Gupta, Shalini Saggu
Jazyk: angličtina
Rok vydání: 2005
Předmět:
Zdroj: Evidence-Based Complementary and Alternative Medicine, Vol 2, Iss 1, Pp 93-97 (2005)
Evidence-based Complementary and Alternative Medicine
ISSN: 1741-4288
Popis: In the present study, oral supplementation ofl-arginine in rats was evaluated for its anti-stress and adaptogenic activity using the cold (5°C)–hypoxia (428 mmHg)–restraint (C-H-R) animal model. A dose-dependent study ofl-arginine was carried out at doses of 12.5, 25.0, 50.0, 100.0, 200.0 and 500.0 mg/kg body weight, administered orally 30 min prior to C-H-R exposure. The time taken by the rat to attain a rectal temperature of 23°C (Trec 23°C) during C-H-R exposure and its recovery toTrec 37°C at normal atmospheric pressure and 32 ± 1°C were used as biomarkers of anti-stress and adaptogenic activity. Biochemical parameters related to lipid peroxidation, anti-oxidants, cell membrane permeability, nitric oxide and stress, with and without administration of the least effectivel-arginine dose, were measured in rats on attainingTrec 23°C andTrec 37°C. The least effective adaptogenic dose ofl-arginine was 100.0 mg/kg body weight. The C-H-R exposure of control rats, on attainingTrec 23°C, resulted in a significant increase in plasma malondialdehyde (MDA), blood lactate dehydrogenase (LDH) and a decrease in blood catalase (CAT) and plasma testosterone levels. On recovery (Trec 37°C) of control rats, there was a further decrease in CAT and plasma testosterone, and an increase in LDH.l-Arginine supplementation resulted in a significant decrease in plasma MDA, an increase in blood superoxide dismutase (SOD), CAT levels maintained at control values and a lower increase in LDH compared with controls (45.3 versus 58.5% and 21.5 versus 105.2%) on attainingTrec 23°C during C-H-R exposure and on recovery toTrec 37°C. The results suggested thatl-arginine possesses potent anti-stress activity during C-H-R exposure and recovery from C-H-R-induced hypothermia.
Databáze: OpenAIRE
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