Ratiometric Detection of Ochratoxin A Using a Regenerable COF-Au-MB-Apt Signal Probe on a Thermal-Regulated Sensor Module

Autor:	Xuan Zhou, Zongbao Sun, Xiaoyu Su, Kaiyi Zheng, Xiaobo Zou, Wen Zhang
Rok vydání:	2023
Předmět:	Analytical Chemistry
Zdroj:	Analytical Chemistry. 95:1916-1923
ISSN:	1520-6882 0003-2700
DOI:	10.1021/acs.analchem.2c04031
Popis:	Ochratoxin A (OTA) frequently contaminates grains and consequently threatens human health. Herein, we develop a regenerable signal probe and apply it to a Au-based screen-printed electrode module (SPE) for OTA determination. The signal probe, containing a structural covalent organic framework, gold nanoparticles (AuNPs), indicative methylene blue (MB), and a highly selective aptamer, is synthesized with hydrothermal and self-assembly methods. The SPE is permanently functionalized with Prussian blue (PB), AuNPs, and semicomplementary ssDNA. The signal probe, absorbed onto this SPE via hybridization, is competitively expelled by OTA, providing a ratiometric readout of Δ
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e4f0034f3250ee425058cebb5f37fa08 https://doi.org/10.1021/acs.analchem.2c04031 Zobrazit plný text záznamu