Gp63 gene polymorphism and population structure of Leishmania donovani complex: influence of the host selection pressure?
| Autor: | N. Seridi, R. Ben Ismail, D. Le Ray, Ikram Guizani, N. Nuwayri-Salti, Jean-Claude Dujardin, Souheila Guerbouj, K. Victoir, M. Belkaid |
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| Přispěvatelé: | Laboratoire d'Epidémiologie et d'Ecologie parasitaire, Institut Pasteur de Tunis, Institut Pasteur de Tunis, Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP), Institute of Tropical Medicine [Antwerp] (ITM), Institut Pasteur d'Algérie, Réseau International des Instituts Pasteur (RIIP), American University of Beirut [Beyrouth] (AUB), This work received Ænancial support from the INCO-DCDGXII-program of the European Community (Contract No. IC18-CT98-0256), from the SERST-PNM96 program (Tunisia) contract No. P96BSP46 and from the Agence Universitaire de le Francophonie (AUPELF-UREF, programme PFR-PDR)., We are grateful to Professor M. E. Wilson, University of Iowa, USA, for kindly providing the gp63 probe of L. chagasi, Professor J. P. Dedet and Dr C. Blaineau, Laboratoire de Parasitologie, Faculte de Medecine, Montpellier, France and Dr J. Alvar, Instituto de Salud Carlos III, Spain, who kindly provided L. donovani and French and Spanish L. infantum parasites, respectively |
| Jazyk: | angličtina |
| Rok vydání: | 2001 |
| Předmět: |
diagnosis
Restriction Mapping Population genetics Protozoology Polymerase Chain Reaction polymorphism 0302 clinical medicine MESH: Metalloendopeptidases/genetics MESH: Antigens Protozoan/genetics Cluster Analysis MESH: Animals Lebanon Leishmania infantum MESH: Phylogeny Phylogeny MESH: Restriction Mapping Genetics 0303 health sciences education.field_of_study biology MESH: Leishmania infantum/genetics Dendrogram Metalloendopeptidases Leishmania chagasi Infectious Diseases Electrophoresis Polyacrylamide Gel France MESH: Algeria MESH: Tunisia Polymorphism Restriction Fragment Length Tunisia 030231 tropical medicine Population Leishmania donovani selection MESH: Selection Genetic Antigens Protozoan MESH: Host-Parasite Interactions Host-Parasite Interactions 03 medical and health sciences Phylogenetics parasitic diseases MESH: Polymorphism Genetic MESH: Spain Animals MESH: Leishmania donovani/genetics [SDV.MP.PAR]Life Sciences [q-bio]/Microbiology and Parasitology/Parasitology MESH: Lebanon Selection Genetic education 030304 developmental biology Polymorphism Genetic MESH: Polymorphism Restriction Fragment Length MESH: Polymerase Chain Reaction/veterinary biology.organism_classification Leishmania MESH: Cluster Analysis immunogen MESH: France Spain Algeria Animal Science and Zoology Parasitology MESH: Electrophoresis Polyacrylamide Gel |
| Zdroj: | Parasitology Parasitology, Cambridge University Press (CUP), 2001, 122 (1), pp.25-35. ⟨10.1017/S0031182000007125⟩ |
| ISSN: | 0031-1820 1469-8161 |
| Popis: | The gp63 encoding genes were characterized by PCR–RFLP in 35 isolates representative of the Leishmania donovani complex (L. infantum, L. donovani, L. archibaldi and L. chagasi), with special attention to Mediterranean L. infantum from different geographical origins, and in separate groups from Old World Leishmania (L. major, L. tropica and L. aethiopica). The aim was to evaluate how the possible selective pressure by the host on these important surface proteins would influence structuring of our sample. Comparison was carried out with the structure obtained (i) from reported isoenzyme data, characters supposed to vary neutrally, and (ii) from PCR–RFLP analysis of gp63 inter-genic regions, containing non-translated spacers and regulatory genes. Polymorphism within the gp63-encoding region, was much higher than in gp63 inter-genic regions. In the gp63 intra-genic dendrogram, the 4 species of L. donovani complex were discriminated and quite distinct from outgroups. Within L. infantum, geographical structuring was observed and did not overlap with the structure built-up from isoenzymes and inter-genic data. These results support the idea of a strong host-selection on gp63, at vector level but most of all at vertebrate (human or dog) immunological level. Furthermore, they illustrate how the nature of genetic characters may influence the perception of population structuring. |
| Databáze: | OpenAIRE |
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