Tailor-made functional surfaces: potential elastomeric biomaterials I
Autor: | R.K.P. Singh, Kashinath R. Patil, Milind Patole, Shrojal M. Desai, Dhananjay Bodas |
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Rok vydání: | 2004 |
Předmět: |
Glycidyl methacrylate
Materials science Biocompatibility Polymers Surface Properties Biomedical Engineering Biophysics Cell Culture Techniques Bioengineering Biocompatible Materials (Hydroxyethyl)methacrylate Models Biological KB Cells Biomaterials Contact angle chemistry.chemical_compound Cell Line Tumor Polymer chemistry Spectroscopy Fourier Transform Infrared Cell Adhesion Humans Cells Cultured Acrylic acid Adhesion chemistry Chemical engineering Elastomers Attenuated total reflection Microscopy Electron Scanning Surface modification Cell Division |
Zdroj: | Journal of biomaterials science. Polymer edition. 14(12) |
ISSN: | 0920-5063 |
Popis: | In the present investigation, different functional monomers, like hydroxyethyl methacrylate, acrylic acid, N-vinyl pyrrolidone and glycidyl methacrylate, have been grafted onto the surface of EPDM film (approx. 200 microm) using simultaneous photo-grafting (lambda > or = 290 nm) and cold plasma-grafting techniques, to alter the surface properties, such as hydrophilicity and, therefore, biocompatibility. Here, we have carried out simultaneous plasma-grafting, unlike the conventional post plasma-grafting. The effect of different surface grafting techniques on the degree of surface modification and resultant biocompatibility has been investigated. The chemical changes on the polymer backbone are followed from the results of attenuated total reflection Fourier transform infrared (ATR-FT-IR) spectroscopy and X-ray photoelectron spectroscopy (XPS), which shows the peaks corresponding to the functional groups of the monomers grafted onto the film surface. The morphology of the modified surfaces was investigated using scanning electron microscopy (SEM) technique. The induced hydrophilicity and resultant cell compatibility were followed from the water contact angle measurements and in vitro human carcinoma cell adhesion/proliferation tests, respectively. All the grafted samples exhibited variable cell compatibilities depending upon the type of monomer and their degree of grafting; however, always better than the neat samples. Hydroxyethyl methacrylate and acrylic acid showed exceptionally high cell compatibility in terms of cell adhesion and proliferation. |
Databáze: | OpenAIRE |
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