Effects of DNA extraction method on Porcine circovirus-2 real-time polymerase chain reaction quantification in swine lymph node samples
| Autor: | Giuliana Franzini, Maria Beatrice Boniotti, Silvia Faccini, Ilaria Barbieri, Carlo Rosignoli, Giovanni Loris Alborali, A. D. Nigrelli |
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| Rok vydání: | 2012 |
| Předmět: |
Circovirus
Swine Real-Time Polymerase Chain Reaction complex mixtures law.invention Porcine Postweaning Multisystemic Wasting Syndrome law medicine Animals Lymph node Polymerase chain reaction Detection limit Chromatography General Veterinary biology Extraction (chemistry) Viral Load biology.organism_classification Molecular biology DNA extraction Porcine circovirus medicine.anatomical_structure Real-time polymerase chain reaction DNA Viral Lymph Nodes Quantitative Real-Time Polymerase Chain Reaction |
| Zdroj: | Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc. 23(6) |
| ISSN: | 1943-4936 |
| Popis: | Quantitative real-time polymerase chain reaction (PCR) has become an important tool for Porcine circovirus-2 (PCV-2) research and diagnosis. However, significant differences in detection limit and quantification data, among laboratories and quantitative real-time PCR methods, have been demonstrated. New efforts are required for providing more accurate and comparable results. The current study is an evaluation of the effects of DNA extraction procedures on PCV-2 quantification in lymph node samples. Differences, greater than 1 log10 copies/g, were shown among PCV-2 loads detected after different extraction procedures. The work highlighted the critical role of the DNA extraction method in PCV-2 quantification by quantitative real-time PCR. This important aspect should be evaluated when comparing data from different laboratories or different studies. The PCV-2 quantification data should not be considered comparable before demonstrating the equivalence of the DNA extraction methods performed. |
| Databáze: | OpenAIRE |
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