Transcriptional analysis of insecticide resistance in Anopheles stephensi using cross-species microarray hybridization

Autor: John Vontas, George K. Christophides, Janet Hemingway, Hilary Ranson, Christos Louis, Jean-Philippe David, Dimitra Nikou
Přispěvatelé: Laboratory of Pesticide Science, Agricultural University of Athens, Institute of Molecular Biology and Biotechnology (IMBB-FORTH), Foundation for Research and Technology - Hellas (FORTH), Laboratoire d'Ecologie Alpine (LECA), Université Joseph Fourier - Grenoble 1 (UJF)-Centre National de la Recherche Scientifique (CNRS)-Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry]), Vector Research Group, Liverpool School of Tropical Medicine (LSTM), Laboratory of Insect Immunogenomics, Division of Cell & Molecular Biology Imperial College London, Department of Biology, University of Crete [Heraklion] (UOC)
Rok vydání: 2007
Předmět:
Microarray
Anopheles gambiae
[SDE.MCG]Environmental Sciences/Global Changes
030231 tropical medicine
Molecular Sequence Data
malaria
[SDV.BID]Life Sciences [q-bio]/Biodiversity
pyrethroid resistance
Insecticide Resistance
03 medical and health sciences
0302 clinical medicine
detoxification chip
Species Specificity
Gene expression
parasitic diseases
Anopheles
Genetics
Animals
Amino Acid Sequence
heterologous hybridization
Molecular Biology
Gene
Anopheles stephensi
030304 developmental biology
DNA Primers
Oligonucleotide Array Sequence Analysis
[SDV.EE]Life Sciences [q-bio]/Ecology
environment
Expressed Sequence Tags
0303 health sciences
Expressed sequence tag
biology
Base Sequence
Gene Expression Profiling
Sequence Analysis
DNA
biology.organism_classification
Molecular biology
3. Good health
Gene expression profiling
Insect Science
Insect Proteins
[SDE.BE]Environmental Sciences/Biodiversity and Ecology
Zdroj: Insect Molecular Biology
Insect Molecular Biology, Wiley, 2007, 16 (3), pp.315-324. ⟨10.1111/j.1365-2583.2007.00728.x⟩
ISSN: 0962-1075
1365-2583
DOI: 10.1111/j.1365-2583.2007.00728.x⟩
Popis: International audience; A large scale microarray (20k MMC1) from the African malaria vector Anopheles gambiae was used to monitor gene expression in insecticide resistant and susceptible strains of the Asian mosquito Anopheles stephensi. Heterologous hybridization at slightly reduced stringency yielded ∼7000 significant signals. Thirty-six putative genes were differentially transcribed between the pyrethroid-resistant (DUB-R) and the susceptible (BEECH) strains. The expression profiles of selected transcripts were verified by real-time PCR. A gene putatively involved in the thickening of the adult cuticle showed the most striking up-regulation in DUB-R. A more specialized microarray containing 231 An. gambiae genes putatively involved in insecticide detoxification was used to further analyse classical insecticide resistance genes. Three glutathione S-transferase (GST) transcripts, one esterase and a cytochrome P450 were up-regulated in the resistant strain, while two peroxidases were down-regulated.
Databáze: OpenAIRE
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