The Identification of Blood Biomarkers of Chronic Neuropathic Pain by Comparative Transcriptomics
| Autor: | David A. Buckley, Barira Islam, Mark I. Johnson, Panagiotis Zis, Bethan Young, David P. Finn, Patrick C McHugh, Helen Radford, John Stephenson, Maurizio Manca |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
medicine.medical_specialty
Neurology Receiver operating characteristic business.industry Chronic pain medicine.disease Gastroenterology Transcriptome Cellular and Molecular Neuroscience Nociception Blood biomarkers Internal medicine Case-Control Studies Neuropathic pain medicine Molecular Medicine Biomarker (medicine) Humans Neuralgia Chronic Pain business Biomarkers |
| Zdroj: | Neuromolecular medicine. 24(3) |
| ISSN: | 1559-1174 |
| Popis: | In this study, we recruited 50 chronic pain (neuropathic and nociceptive) and 43 pain-free controls to identify specific blood biomarkers of chronic neuropathic pain (CNP). Affymetrix microarray was carried out on a subset of samples selected 10 CNP and 10 pain-free control participants. The most significant genes were cross-validated using the entire dataset by quantitative real-time PCR (qRT-PCR). In comparative analysis of controls and CNP patients, WLS (P = 4.80 × 10–7), CHPT1 (P = 7.74 × 10–7) and CASP5 (P = 2.30 × 10–5) were highly significant, whilst FGFBP2 (P = 0.00162), STAT1 (P = 0.00223), FCRL6 (P = 0.00335), MYC (P = 0.00335), XCL2 (P = 0.0144) and GZMA (P = 0.0168) were significant in all CNP patients. A three-arm comparative analysis was also carried out with control as the reference group and CNP samples differentiated into two groups of high and low S-LANSS score using a cut-off of 12. STAT1, XCL2 and GZMA were not significant but KIR3DL2 (P = 0.00838), SH2D1B (P = 0.00295) and CXCR31 (P = 0.0136) were significant in CNP high S-LANSS group (S-LANSS score > 12), along with WLS (P = 8.40 × 10–5), CHPT1 (P = 7.89 × 10–4), CASP5 (P = 0.00393), FGFBP2 (P = 8.70 × 10–4) and FCRL6 (P = 0.00199), suggesting involvement of immune pathways in CNP mechanisms. None of the genes was significant in CNP samples with low (MYC, STAT1, TLR4, CASP5 and WLS gene expression could be potentially used as a biomarker signature of CNP (AUROC − 0.852, (0.773, 0.931 95% CI)). |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full text from SpringerLink