The Anaerobic Product Ethanol Promotes Autophagy-Dependent Submergence Tolerance in Arabidopsis

Autor: Ning Zhai, Li-Bing Yuan, Li-Juan Xie, Shan Shan Zhao, Ying Zhou, Lu-Jun Yu, Liang Chen, Shi Xiao, Li-Li Shi
Jazyk: angličtina
Rok vydání: 2020
Předmět:
0106 biological sciences
0301 basic medicine
autophagy
Cellular respiration
Cell Respiration
Mutant
Arabidopsis
01 natural sciences
Article
Catalysis
Green fluorescent protein
lcsh:Chemistry
Inorganic Chemistry
03 medical and health sciences
Gene Expression Regulation
Plant
Immersion
Humans
Anaerobiosis
Physical and Theoretical Chemistry
lcsh:QH301-705.5
Molecular Biology
Spectroscopy
Alcohol dehydrogenase
chemistry.chemical_classification
Reactive oxygen species
submergence
biology
Arabidopsis Proteins
Chemistry
hypoxia
ADH1
Organic Chemistry
Autophagy
General Medicine
biology.organism_classification
Computer Science Applications
Cell biology
Oxygen
030104 developmental biology
lcsh:Biology (General)
lcsh:QD1-999
Seedlings
biology.protein
Fermentation
ethanol
Reactive Oxygen Species
010606 plant biology & botany
Zdroj: International Journal of Molecular Sciences
Volume 21
Issue 19
International Journal of Molecular Sciences, Vol 21, Iss 7361, p 7361 (2020)
ISSN: 1422-0067
DOI: 10.3390/ijms21197361
Popis: In response to hypoxia under submergence, plants switch from aerobic respiration to anaerobic fermentation, which leads to the accumulation of the end product, ethanol. We previously reported that Arabidopsis thaliana autophagy-deficient mutants show increased sensitivity to ethanol treatment, indicating that ethanol is likely involved in regulating the autophagy-mediated hypoxia response. Here, using a transcriptomic analysis, we identified 3909 genes in Arabidopsis seedlings that were differentially expressed in response to ethanol treatment, including 2487 upregulated and 1422 downregulated genes. Ethanol treatment significantly upregulated genes involved in autophagy and the detoxification of reactive oxygen species. Using transgenic lines expressing AUTOPHAGY-RELATED PROTEIN 8e fused to green fluorescent protein (GFP-ATG8e), we confirmed that exogenous ethanol treatment promotes autophagosome formation in vivo. Phenotypic analysis showed that deletions in the alcohol dehydrogenase gene in adh1 mutants result in attenuated submergence tolerance, decreased accumulation of ATG proteins, and diminished submergence-induced autophagosome formation. Compared to the submergence-tolerant Arabidopsis accession Columbia (Col-0), the submergence-intolerant accession Landsberg erecta (Ler) displayed hypersensitivity to ethanol treatment
we linked these phenotypes to differences in the functions of ADH1 and the autophagy machinery between these accessions. Thus, ethanol promotes autophagy-mediated submergence tolerance in Arabidopsis.
Databáze: OpenAIRE
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