Multi-sulfonated ligands on gold nanoparticles as virucidal antiviral for Dengue virus
| Autor: | Ozgun Kocabiyik, Quy Khac Ong, Danilo Migoni, Dariusz Witt, Valentino Laquintana, Piotr Barski, Francesco Stellacci, Antonella Zacheo, Jan Weber, Nunzio Denora, Giuseppe Felice Mangiatordi, Jan Hodek, Nicoletta Depalo, Silke Krol, Elisabetta Fanizza |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
medicine.medical_treatment lcsh:Medicine Metal Nanoparticles 02 engineering and technology Dengue virus medicine.disease_cause Ligands Antiviral Agents Article Cell Line Dengue 03 medical and health sciences Tissue culture Cell Line Tumor inhibitors Chlorocebus aethiops medicine Animals Humans lcsh:Science Vero Cells EC50 search Multidisciplinary Protease Ligand Chemistry Drug discovery Infectious dose lcsh:R protease Hep G2 Cells Dengue Virus 021001 nanoscience & nanotechnology Virology polymerase Molecular Docking Simulation 030104 developmental biology Nanomedicine Colloidal gold Viral infection Toxicity Molecular docking Hepatocytes entry lcsh:Q Gold 0210 nano-technology |
| Zdroj: | Scientific Reports Scientific reports (Nature Publishing Group) 10 (2020). doi:10.1038/s41598-020-65892-3 info:cnr-pdr/source/autori:Zacheo, Antonella; Hodek, Jan; Witt, Dariusz; Mangiatordi, Giuseppe F. Elice; Ong, Quy K.; Kocabiyik, Ozgun; Depalo, Nicoletta; Fanizza, Elisabetta; Laquintana, Valentino; Denora, Nunzio; Migoni, Danilo; Barski, Piotr; Stellacci, Francesco; Weber, Jan; Krol, Silke/titolo:Multi-sulfonated ligands on gold nanoparticles as virucidal antiviral for Dengue virus/doi:10.1038%2Fs41598-020-65892-3/rivista:Scientific reports (Nature Publishing Group)/anno:2020/pagina_da:/pagina_a:/intervallo_pagine:/volume:10 Scientific Reports, Vol 10, Iss 1, Pp 1-9 (2020) |
| ISSN: | 2045-2322 |
| Popis: | Dengue virus (DENV) causes 390 million infections per year. Infections can be asymptomatic or range from mild fever to severe haemorrhagic fever and shock syndrome. Currently, no effective antivirals or safe universal vaccine is available. In the present work we tested different gold nanoparticles (AuNP) coated with ligands ω-terminated with sugars bearing multiple sulfonate groups. We aimed to identify compounds with antiviral properties due to irreversible (virucidal) rather than reversible (virustatic) inhibition. The ligands varied in length, in number of sulfonated groups as well as their spatial orientation induced by the sugar head groups. We identified two candidates, a glucose- and a lactose-based ligand showing a low EC50 (effective concentration that inhibit 50% of the viral activity) for DENV-2 inhibition, moderate toxicity and a virucidal effect in hepatocytes with titre reduction of Median Tissue Culture Infectious Dose log10TCID50 2.5 and 3.1. Molecular docking simulations complemented the experimental findings suggesting a molecular rationale behind the binding between sulfonated head groups and DENV-2 envelope protein. |
| Databáze: | OpenAIRE |
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