New Bacterial Surface Display System Development and Application Based on Bacillus subtilis YuaB Biofilm Component as an Anchoring Motif
| Autor: | Woo-Il Kim, Daeun Kim, June-Hyung Kim |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
0106 biological sciences
bacterial surface display system Biomedical Engineering Bioengineering Bacillus subtilis 01 natural sciences Applied Microbiology and Biotechnology Bacterial cell structure law.invention Cell wall 03 medical and health sciences law 010608 biotechnology 030304 developmental biology 0303 health sciences biology Strain (chemistry) Chemistry flow cytometry fungi Biofilm Protoplast biology.organism_classification Membrane protoplast Recombinant DNA Biophysics YuaB Research Paper Biotechnology |
| Zdroj: | Biotechnology and Bioprocess Engineering |
| ISSN: | 1976-3816 1226-8372 |
| DOI: | 10.1007/s12257-020-0397-7 |
| Popis: | Bacterial surface display system has been adopted in various biotechnological applications. In the case of Bacillus subtilis, most of the studies have been developed using spore based surface display system utilizing the inherent rigidity of spore against heat, alkali, and shear stress. But, spore harvest, purification and separation need additional cost and labor. To eliminate this procedure and to use the gram-positive nature of B. subtilis, YuaB, which is one of the major B. subtilis biofilm components and locates in the cell wall, based cell surface display system, is developed. P43 promoter driven overexpression of YuaB-His6 tag does not hamper bacterial cell growth and promoted biofilm formation of recombinant strain. Flow cytometry of recombinant strain and its protoplast using FITC-Anti His6 antibody, verified that YuaB locate in plasma membrane and protrude to the outside of cell wall, which means YuaB can be used as very efficient anchoring motif. Using surface expressed YuaB-His6 tag, removal of divalent metal ion, Cu2+ and Ni2+, was tried to test its possibility for the environmental application of developed system. |
| Databáze: | OpenAIRE |
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