Role of endocytosis and cathepsin-mediated activation in Nipah virus entry
| Autor: | Lena Thiel, Andrea Maisner, Sandra Diederich |
|---|---|
| Jazyk: | angličtina |
| Předmět: |
Endosome
viruses Ephrin-B2 Nipah virus Biology Endocytosis Article Cathepsin B Cell Line Cathepsin L Cell membrane Virus entry Viral Envelope Proteins Viral entry Virology Cathepsin cleavage medicine Animals Humans Henipavirus Infections Cathepsin Cell Membrane Virus Internalization Fusion protein medicine.anatomical_structure biology.protein Receptors Virus |
| Zdroj: | Virology |
| ISSN: | 0042-6822 |
| DOI: | 10.1016/j.virol.2008.02.019 |
| Popis: | The recent discovery that the Nipah virus (NiV) fusion protein (F) is activated by endosomal cathepsin L raised the question if NiV utilize pH- and protease-dependent mechanisms of entry. We show here that the NiV receptor ephrin B2, virus-like particles and infectious NiV are internalized from the cell surface. However, endocytosis, acidic pH and cathepsin-mediated cleavage are not necessary for the initiation of infection of new host cells. Our data clearly demonstrate that proteolytic activation of the NiV F protein is required before incorporation into budding virions but not after virus entry. |
| Databáze: | OpenAIRE |
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