Characterisation of the external surfaces of Pseudomonas aeruginosa isolated from human blood and respiratory tract
| Autor: | Y. Niho, Kyoko Okada, Y. Sawae, Akiko Umeda, Hiroyasu Misumi, Kazunobu Amako, Katsuhiko Eguchi |
|---|---|
| Rok vydání: | 1994 |
| Předmět: |
Lipopolysaccharides
Microbiology (medical) Blood Bactericidal Activity Immunoblotting Bacteremia Biology medicine.disease_cause Microbiology Freezing medicine Humans Pseudomonas Infections Microscopy Immunoelectron Respiratory Tract Infections Fibrous layer Human blood Pseudomonas aeruginosa Sputum General Medicine Microscopy Electron medicine.anatomical_structure Electrophoresis Polyacrylamide Gel medicine.symptom Respiratory tract |
| Zdroj: | Journal of Medical Microbiology. 40:282-287 |
| ISSN: | 1473-5644 0022-2615 |
| DOI: | 10.1099/00222615-40-4-282 |
| Popis: | Summary The surface structures of the cell envelopes of 16 clinical isolates of Pseudomonas aeruginosa were examined by electronmicroscopy with the new fixation technique of freezesubstitution. Two types of structures were observed among the organisms. In one group of strains, mostly isolated from blood, a dense fibrous layer c. 30 nm thick was found around the outer-membrane surface, whereas no such structure was observed in the other group of isolates, most of which were from sputum. Lipopolysaccharides extracted from the isolates with a dense fibrous layer were found by SDS-PAGE to have long O-polysaccharide chains, whereas strains without such a layer mostly had lipopolysaccharides that lacked high mol. wt. O-polysaccharide chains. |
| Databáze: | OpenAIRE |
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