Deep Learning-Based Nuclear Lobe Count Method for Differential Count of Neutrophils
| Autor: | Haruhi Ida, Keiko Miwa, Mayu Yabuta, Hiromi Masauzi, Kazunori Okada, Nobuo Masauzi, Sanae Kaga, Iori Nakamura |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
white blood cell morphology
Pathology medicine.medical_specialty Neutrophils General Biochemistry Genetics and Molecular Biology Giemsa stain computer vision Sepsis convolutional neural networks medicine Humans Megaloblastic anemia business.industry Deep learning deep learning General Medicine medicine.disease blood cell automatic image analysis Peripheral blood Lobe medicine.anatomical_structure Feature (computer vision) Neural Networks Computer Artificial intelligence Abnormality business |
| Zdroj: | The Tohoku journal of experimental medicine. 254(3):199-206 |
| ISSN: | 0040-8727 |
| Popis: | Differentiating neutrophils based on the count of nuclear lobulation is useful for diagnosing various hematological disorders, including megaloblastic anemia, myelodysplastic syndrome, and sepsis. It has been reported that one-fifth of sepsis-infected patients worldwide died between 1990 and 2017. Notably, fewer nuclear-lobed and stab-formed neutrophils develop in the peripheral blood during sepsis. This abnormality can serve as an early diagnostic criterion. However, testing this feature is a complex and time-consuming task that is rife with human error. For this reason, we apply deep learning to automatically differentiate neutrophil and nuclear lobulation counts and report the world's first small-scale pilot. Blood films are prepared using venous peripheral blood taken from four healthy volunteers and are stained with May-Grünwald Giemsa stain. Six-hundred 360 × 363-pixel images of neutrophils having five different nuclear lobulations are automatically captured by Cellavision DM-96, an automatic digital microscope camera. Images are input to an original architecture with five convolutional layers built on a deep learning neural-network platform by Sony, Neural Network Console. The deep learning system distinguishes the four groups (i.e., band-formed, two-, three-, and four- and five- segmented) of neutrophils with up to 99% accuracy, suggesting that neutrophils can be automatically differentiated based on their count of segmented nuclei using deep learning. |
| Databáze: | OpenAIRE |
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