CD44 induces FOXP3 expression and is related with favorable outcome in breast carcinoma
| Autor: | F. I. Aranda, Fernando Ortiz-Martínez, Montserrat Rodrigo-Baños, Enrique Lerma, Gloria Peiró, Eloy Pomares-Navarro, Elena Sanmartín, Araceli García-Martínez, Pascual Martínez-Peinado, José M. Sempere-Ortells, Marta García-Escolano, Leire Andrés |
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| Přispěvatelé: | Universidad de Alicante. Departamento de Biotecnología, Grupo de Inmunología, Biología Celular y del Desarrollo |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Adult Male FOXP3 Breast carcinoma Inmunología chemical and pharmacologic phenomena Breast Neoplasms T-Lymphocytes Regulatory Disease-Free Survival Pathology and Forensic Medicine 03 medical and health sciences Young Adult 0302 clinical medicine Breast cancer Gene expression medicine Biomarkers Tumor Humans CD44 Molecular Biology Survival analysis Aged Aged 80 and over Messenger RNA biology mRNA expression hemic and immune systems Forkhead Transcription Factors Cell Biology General Medicine Middle Aged medicine.disease Prognosis Immunohistochemistry 030104 developmental biology Hyaluronan Receptors 030220 oncology & carcinogenesis biology.protein Cancer research Female Neoplasm Recurrence Local Breast carcinoma CD44 FOXP3 Prognosis mRNA expression |
| Zdroj: | VIRCHOWS ARCH r-FISABIO. Repositorio Institucional de Producción Científica instname r-FISABIO: Repositorio Institucional de Producción Científica Fundación para el Fomento de la Investigación Sanitaria y Biomédica de la Comunitat Valenciana (FISABIO) VIRCHOWS ARCHIV r-ISABIAL. Repositorio Institucional de Producción Científica del Instituto de Investigación Biomédica y Sanitaria de Alicante r-IIB SANT PAU. Repositorio Institucional de Producción Científica del Instituto de Investigación Biomédica Sant Pau |
| ISSN: | 0945-6317 |
| Popis: | We studied the relationship between CD44 and Forkhead box P3 (FOXP3) gene expression in cell lines and breast carcinomas and their association with clinicopathological variables and patient outcome. We assessed messenger RNA (mRNA) expression of CD44 and FOXP3 by quantitative real-time PCR and determined the number of FOXP3+ Tregs by immunohistochemistry in 264 breast cancer specimens. CD44 was stimulated with hyaluronan treatment, and the accompanying changes in FOXP3 mRNA expression in breast cancer cell lines representing breast cancer subtype were assessed. We found that lower CD44 expression correlated with the presence of necrosis, lymph-vascular invasion, grade 3 tumors, and aggressive phenotype (HER2 and basal-like). FOXP3 mRNA correlated positively with CD44 mRNA expression and Treg content. Moreover, stimulation of CD44 expression by hyaluronan in cell lines increased FOXP3 expression, which supports that their regulation is associated. Survival analysis revealed that low CD44 expression is associated with higher frequency of recurrence. Our findings indicate that CD44 has a regulatory role in FOXP3 expression and is associated with good prognostic factors in breast cancer. This study was supported by grants from the Instituto de Salud Carlos III (FIS PI10/00082), Conselleria de Sanitat-Generalitat Valenciana (AP-172/10), and Fundación de la Comunidad Valenciana para la Investigación, FCVI-HGUA (2011/PC-03; UGP-14-271). |
| Databáze: | OpenAIRE |
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