Aminoglycoside-induced increase of intracellular calcium in LLC-PK1 cells due to an artifact caused by trypsin and EDTA
Autor: | D. N. Gilbert, R. A. Land, S J Kohlhepp, K. Hermsmeyer |
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Rok vydání: | 1994 |
Předmět: |
medicine.medical_specialty
Swine chemistry.chemical_element Video microscopy Calcium Biology Calcium in biology Cell Line Nephrotoxicity Norepinephrine Internal medicine Image Processing Computer-Assisted medicine Animals Trypsin Pharmacology (medical) Edetic Acid Pharmacology Aminoglycoside Anti-Bacterial Agents Infectious Diseases Endocrinology Microscopy Fluorescence chemistry Cell culture Tobramycin Gentamicins Intracellular Research Article medicine.drug |
Zdroj: | Antimicrobial Agents and Chemotherapy. 38:1065-1070 |
ISSN: | 1098-6596 0066-4804 |
DOI: | 10.1128/aac.38.5.1065 |
Popis: | Dietary calcium supplements attenuate experimental aminoglycoside nephrotoxicity. In cultured renal tubular cells, intracellular calcium levels have been reported to rise with aminoglycoside addition to the culture medium. In experiments designed to verify the in vitro influence of calcium on cultured kidney cells, we detected an unexpected artifact. When we resuspended cultured LLC-PK1 cells with trypsin and EDTA to measure intracellular calcium levels, our results correlated well with previously reported values. However, we saw no increase in intracellular calcium levels when we measured them by digital imaging video microscopy unless trypsin-EDTA exposure preceded aminoglycoside exposure. This apparent artifact should be considered in any study of the effects of various agents on intracellular calcium levels. |
Databáze: | OpenAIRE |
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