Effects of recombinant-hemoglobin solutions rHb2.0 and rHb1.1 on blood pressure, intestinal blood flow, and gut oxygenation in a rat model of hemorrhagic shock
| Autor: | Jing-Feng Liu, Jan Klein, Nicolaas J.H. Raat, Can Ince, Michael P. Doyle, Kenneth E. Burhop |
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| Přispěvatelé: | Biomedical Engineering and Physics, Translational Physiology, Anesthesiology, Surgery |
| Jazyk: | angličtina |
| Rok vydání: | 2005 |
| Předmět: |
Oncotic pressure
Male medicine.medical_specialty Mean arterial pressure Resuscitation Hemoglobins Abnormal Blood Pressure Shock Hemorrhagic Nitric Oxide Pathology and Forensic Medicine Hemoglobins Mesenteric Veins Blood Substitutes Internal medicine medicine Animals Splanchnic Circulation Rats Wistar business.industry Hemodynamics General Medicine Oxygenation Free Radical Scavengers Recombinant Proteins Mesenteric Arteries Rats Oxygen Disease Models Animal Blood pressure medicine.anatomical_structure Vasoconstriction Shock (circulatory) Anesthesia Vascular resistance Cardiology medicine.symptom business |
| Zdroj: | Journal of laboratory and clinical medicine, 145(1), 21-32. Mosby Inc. Journal of Laboratory and Clinical Medicine, 145, 21-32. Mosby Inc. |
| ISSN: | 0022-2143 |
| Popis: | The vasoconstriction induced by hemoglobin-based oxygen carriers (HBOCs), mainly a result of nitric oxide (NO) scavenging, until now has limited the application of HBOCs as resuscitation fluids. In this study, we tested the hypothesis that the new modified recombinant-hemoglobin solution rHb2.0, with a 20 to 30 times lesser NO-scavenging rate, would minimize vasoconstriction without adverse effects on microvascular oxygenation. Responses were compared with those to rHb1.1, a recombinant-hemoglobin solution with a wild-type NO-scavenging rate, as well as an oncotically matched albumin solution. In a fixed-pressure (40 mm Hg) rat model of hemorrhagic shock and resuscitation, rHb2.0 and albumin both restored mean arterial pressure (MAP) to baseline values, whereas rHb1.1 increased MAP to 27% above the baseline value. Mesenteric vascular resistance after resuscitation with rHb2.0 was 57% less than that with rHb1.1. rHb2.0 was found to have 55% greater intestinal oxygen delivery (Do 2int ) and resulted in a 27% lower oxygen-extraction rate than did rHb1.1 after resuscitation. Intestinal microvascular Po 2 , determined on the basis of oxygen-dependent quenching of palladium-porphyrin phosphorescence, revealed no difference between rHb2.0 and rHb1.1. The findings of this study confirm that the well-known pressure effect of HBOCs is caused by their effect on the NO-scavenging rate; recombinant modification of this rate did not increase MAP during resuscitation compared with baseline values. Although systemic vasoconstriction was absent, intestinal vasoconstriction almost negligible, and Do 2int greater after resuscitation with rHb2.0, the effect of rHb2.0 on pH, base-excess and microvascular Po 2 levels after resuscitation were comparable to those achieved with the use of the albumin solution. |
| Databáze: | OpenAIRE |
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