Abnormal B-cell Regulation in Highly Sensitized Patients with Sustained Serum Levels of Antibody to HLA Class I Antigens
| Autor: | Nashrudeen Hack, Sarita Angra, Neal den Hollander, Carl J. Cardella, Theresa L McKnight |
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| Rok vydání: | 2005 |
| Předmět: |
Male
Human leukocyte antigen Antibodies Highly sensitized Immunoglobulin Idiotypes Humans Medicine B cell Sensitization Cell Proliferation B-Lymphocytes Transplantation biology business.industry Cell growth Histocompatibility Antigens Class I Molecular biology In vitro Blood medicine.anatomical_structure Immunization Immunoglobulin G Immunology biology.protein Female Antibody business |
| Zdroj: | Transplantation. 80:1226-1232 |
| ISSN: | 0041-1337 |
| DOI: | 10.1097/01.tp.0000179640.06825.bf |
| Popis: | Background. There are anti-idiotypes in the sera of highly sensitized (HS) patients that stimulate B cells to produce antibody to HLA class I antigens. The purpose of this study is to determine if there is an abnormality in B cell responses to these anti-idiotypes. Methods. Supernatants from normal and HS B cells exposed to either HLA-like anti-idiotypes or HS sera were tested for IgG and antibody to HLA class I antigens by ELISA and flow beads. Results. When stimulated with HS sera, HS B cells produced antibody to HLA class I antigens (in vitro) (12/12) but normal B cells did not (0/10) (P |
| Databáze: | OpenAIRE |
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