Large Scale Preparation of Liver Mitochondria with the Waring Blendor

Autor:	Mary A. Cottone, Walter J. Pories, Robert F. Witter
Rok vydání:	1953
Předmět:	chemistry.chemical_classification Differential centrifugation Oxidase test Sucrose Chromatography Fatty acid Mitochondria Liver Mitochondrion General Biochemistry Genetics and Molecular Biology Mitochondria chemistry.chemical_compound Liver chemistry Biochemistry Isotonic Homogenizer Motor speed
Zdroj:	Experimental Biology and Medicine. 84:674-677
ISSN:	1535-3699 1535-3702
DOI:	10.3181/00379727-84-20748
Popis:	SummaryA method is described for the preparation of mitochondria from 50 to 100 g of liver. The tissue is pulped in a special press and homogenized at a reduced motor speed for a short time in the Waring blendor in isotonic sucrose. The mitochondria are isolated by differential centrifugation from isotonic sucrose. The ATP-ase, fatty acid oxidase, and succinoxidase activities of these preparations were shown to be similar to those of mitochondria prepared with a pestle type homogenizer.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e4cdde4e3a62f00e5c6846ac278fe2db https://doi.org/10.3181/00379727-84-20748 Zobrazit plný text záznamu