The Ultrashort Peptide OW: A New Antibiotic Adjuvant
| Autor: | Ahmad Abualhaijaa, Mohammad Alsaggar, Majed M Masadeh, Yara Al Tall, Mohammed T Qaoud, Karem H. Alzoubi |
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| Rok vydání: | 2019 |
| Předmět: |
Methicillin-Resistant Staphylococcus aureus
0301 basic medicine medicine.drug_class 030106 microbiology Antibiotics Antimicrobial peptides Pharmaceutical Science Microbial Sensitivity Tests medicine.disease_cause Microbiology 03 medical and health sciences Antibiotic resistance Drug Resistance Multiple Bacterial Ampicillin medicine Humans Adjuvants Pharmaceutic Chemistry Pseudomonas aeruginosa Drug Synergism Antimicrobial Methicillin-resistant Staphylococcus aureus Anti-Bacterial Agents HEK293 Cells 030104 developmental biology Biofilms Oligopeptides Rifampicin Biotechnology medicine.drug |
| Zdroj: | Current Pharmaceutical Biotechnology. 20:745-754 |
| ISSN: | 1389-2010 |
| Popis: | Background:The over use of current antibiotics and low discovery rate of the new ones are leading to rapid development of multidrug-resistant pathogens worldwide. Antimicrobial peptides have shown promising results against multidrug-resistant bacteria.Objective:To investigate the antimicrobial activity of a new ultrashort hexapeptide (OW).Methods:The OW hexapeptide was designed and tested against different strains of bacteria with different levels of sensitivity. Bacterial susceptibility assays were performed according to the guidelines of the Clinical and Laboratory Institute (CLSI). The synergistic studies were then conducted using the Checkerboard assay. This was followed by checking the hemolytic effect of the hexapeptide against human blood cells and Human Embryonic Kidney cell line (HEK293). Finally, the antibiofilm activities of the hexapeptide were studied using the Biofilm Calgary method.Results:Synergistic assays showed that OW has synergistic effects with antibiotics of different mechanisms of action. It showed an outstanding synergism with Rifampicin against methicillin resistant Staphylococcus aureus; ΣFIC value was 0.37, and the MIC value of Rifampicin was decreased by 85%. OW peptide also displayed an excellent synergism with Ampicillin against multidrug-resistant Pseudomonas aeruginosa, with ΣFIC value of less than 0.38 and a reduction of more than 96% in the MIC value of Ampicillin.Conclusion:This study introduced a new ultrashort peptide (OW) with promising antimicrobial potential in the management of drug-resistant infectious diseases as a single agent or in combination with commonly used antibiotics. Further studies are needed to investigate the exact mechanism of action of these peptides. |
| Databáze: | OpenAIRE |
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